Structural characterization and in vitro processing of Escherichia coli ribosomal RNA transcripts containing 5- triphosphates, leader sequences, 16 S rRNA, and spacer tRNAs.
Structural characterization and in vitro processing of Escherichia coli ribosomal RNA transcripts containing 5- triphosphates, leader sequences, 16 S rRNA, and spacer tRNAs.
复制标题
含有 5-三磷酸、前导序列、16 S rRNA 和间隔 tRNA 的大肠杆菌核糖体 RNA 转录物的结构表征和体外加工。
DOI：
10.1016/0022-2836(80)90188-6
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发表时间：
1980
影响因子：
5.6
通讯作者：
Apirion,D
中科院分区：
文献类型：
--
作者：
Gegenheimer,P;Apirion,D
与核糖体RNA加工内切酶RNAase III相碰撞的埃希氏菌菌株产生18s RNA，其中包含p16前体rRNA的整个序列以及在5 '端额外的“先导”序列。RNAase III−RNAase P−细胞合成一种19s RNA，其中包括p16rrna加上leader序列和transfer RNA序列。19个S RNA转录本来自大部分或全部rRNA基因簇。它们以ATP或GTP开始以tRNA序列结束。RNAase P切割19s RNA产生成熟的tRNA2Glu、tRNA1Ile和tRNA1BAla，即所谓的“间隔”转移RNA，它们是从16s和23s rRNA反式子之间的间隔区转录而来。通过RNAase III切割19s RNA产生p16 rRNA、前导RNA和间隔RNA片段。先导rna由ATP或GTP起始。间隔片段从p16 rRNA的3 ‘端延伸到间隔tRNA的成熟3 ’端；随后用RNAase P处理释放成熟的间隔rna。这些结果表明，RNAase III是第一个加工新生rRNA转录本5′区的酶，间隔tRNA序列位于19个S RNA分子的3′端，tRNA加工酶RNAase P参与了rRNA操作子转录本的加工。利用这些数据，可以绘制至少五个不同核糖体RNA操纵子近端区域的转录本的加工切割图。
Strains ofEscherichia colilacking the ribosomal RNA processing endonuclease RNAase III produce an 18 S RNA species which contains the entire sequence of p16 precursor rRNA plus additional “leader” sequences at the 5′ end. RNAase III−RNAase P−cells synthesize a 19 S RNA which includes p16 rRNA plus leader sequences and transfer RNA sequences. The 19 S RNA transcripts originate from most or all of the rRNA gene clusters. They start with ATP or GTP and end with tRNA sequences.RNAase P cleavage of 19 S RNA processes out mature tRNA2Glu, tRNA1Ile, and tRNA1BAla, the so-called “spacer” transfer RNAs which are transcribed from the spacer region between 16 S and 23 S rRNA cistrons. Cleavage of 19 S RNA by RNAase III produces p16 rRNA, leader RNAs, and spacer RNA fragments. Leader RNAs are initiated with ATP or GTP. The spacer fragments extend from the 3′ terminus of p16 rRNA to the mature 3′ terminus of a spacer tRNA; subsequent treatment with RNAase P releases mature spacer tRNAs. These results indicate that RNAase III is the first enzyme to process the 5′ region of nascent rRNA transcripts, that spacer tRNA sequences are located at the 3′ end of 19 S RNA molecules, and that the tRNA processing enzyme RNAase P participates in the processing of transcripts from rRNA operons. Using this data, a processing cleavage map can be drawn of transcripts from the proximal regions of at least five different ribosomal RNA operons.