Sex Differences in Experimentally Induced Colitis in Mice: a Role for Estrogens
Sex Differences in Experimentally Induced Colitis in Mice: a Role for Estrogens
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DOI：
10.1007/s10753-015-0180-7
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发表时间：
2015-10-01
期刊：
影响因子：
5.1
通讯作者：
Celec, Peter
中科院分区：
文献类型：
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作者：
Babickova, Janka;Tothova, L'ubomira;Celec, Peter
炎症性肠病(IBD)的发病率和进展存在性别差异，如溃疡性结肠炎和克罗恩病。已报道的观察性研究的差异是有争议的，性激素在IBD发病机制中的作用也不清楚。这项研究的目的是分析实验性结肠炎进展过程中的性别差异。在饮用水中加入2%葡聚糖硫酸钠(DSS)诱导成年小鼠实验性结肠炎。雄性和雌性小鼠作为正常小鼠，去性腺，并补充雌二醇或睾酮。与雄鼠相比，雌性诱导性结肠炎小鼠的结肠明显更长(p<；0.001)，体重下降更低(p<；0.001)，大便一致性分数也更低(p<；0.05)。组织病理学分析显示，雌性小鼠的炎性浸润物(P<；0.001)和隐窝损伤(P<；0.001)较少。患结肠炎的雌性小鼠结肠匀浆中的肿瘤坏死因子-α浓度也较低(p<；0.01)。在去卵巢的小鼠中补充雌二醇可以改善结肠炎的严重程度。雌性小鼠对化学诱导的结肠炎有部分保护作用。这种保护似乎是由雌二醇所介导的。
Sex differences have been found in the incidence and progression of inflammatory bowel diseases (IBD) such as ulcerative colitis and Crohn's disease. The reported differences in observational studies are controversial, and the effects of sex hormones on the pathogenesis of IBD are not clear. The aim of this study was to analyze sex differences in the progression of experimentally induced colitis. Experimental colitis was induced in adult mice by adding 2 % dextran sodium sulfate (DSS) into drinking water. Male and female mice were used as intact, gonadectomized, and supplemented with either estradiol or testosterone. In comparison to males, female mice with induced colitis had significantly longer colon (p < 0.05), lower decrease in body weight (p < 0.001), and lower stool consistency score (p < 0.05). Histopathological analysis showed less inflammatory infiltrates (p < 0.001) and crypt damage (p < 0.001) in female mice. Female mice with colitis had also lower concentration of TNF-alpha in colon homogenates (p < 0.01). Supplementation with estradiol in ovariectomized mice ameliorated the severity of colitis. Female mice are partially protected against chemically induced colitis. This protection seems to be mediated by estradiol.