Reflecting time-of-flight mass spectrometer with an electrospray ion source and orthogonal extraction.
Reflecting time-of-flight mass spectrometer with an electrospray ion source and orthogonal extraction.
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DOI：
10.1021/ac00073a022
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发表时间：
1994
影响因子：
7.4
通讯作者：
A. Verentchikov;W. Ens;K. Standing
中科院分区：
文献类型：
--
作者：
A. Verentchikov;W. Ens;K. Standing
一个电喷雾源已经连接到一个反射式飞行时间质谱仪。离子以连续的束流形式沿垂直于光谱仪轴线的方向进入，并通过施加到注入电极的电脉冲形成速度平行于光谱仪轴线的短的离子爆发。该仪器可以在线性模式下工作，在静电镜后面检测到离子，或者在反射模式下工作，在反射后检测到离子。在后一种情况下，对于m/z约600，时间分辨率为<；或=8 ns，能够观察到高达约4000u的质量的单个同位素峰。灵敏度足以测量10fmol细胞色素c(约12000u)的质谱图。该光谱仪不限制m/z值的范围，已观察到的离子最高可达m/z约6000。在适当调整源中的pH和其他条件下，电离非常软，能够注入弱结合的络合物；这些已在光谱仪中加速并测量，没有观察到碎裂。
An electrospray source has been coupled to a reflecting time-of-flight mass spectrometer. The ions enter as a continuous beam in a direction perpendicular to the spectrometer axis and are formed into short bursts of ions with velocities parallel to the axis by electrical pulses applied to injection electrodes. The instrument may be operated either in the linear mode, with ions detected behind the electrostatic mirror, or in the reflecting mode, with ions detected after reflection. In the latter case the time resolution is < or = 8 ns for m/z approximately 600, enabling observation of individual isotopic peaks for masses up to about 4000 u. The sensitivity is adequate to enable measurement of mass spectra for 10 fmol of cytochrome c (approximately 12,000 u). The spectrometer does not limit the range of m/z values, and ions have been observed up to m/z approximately 6000. With proper adjustment of pH and other conditions in the source, the ionization is very soft, enabling injection of weakly bound complexes; these have been accelerated and measured in the spectrometer without observable fragmentation.