Development and trait evaluation of chromosome single-segment substitution lines of O. meridionalis in the background of O. sativa
Development and trait evaluation of chromosome single-segment substitution lines of O. meridionalis in the background of O. sativa
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苜蓿背景下南方稻染色体单片段替换系的发育及性状评价
DOI：
10.1007/s10681-017-2072-4
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发表时间：
2017-12-01
期刊：
影响因子：
1.9
通讯作者：
Fu, Xuelin
中科院分区：
文献类型：
--
作者：
He, Ning;Wu, Rouxian;Fu, Xuelin
南方稻(O. meridionalis)是稻属AA基因组的野生物种，含有大量对栽培稻改良有益的基因。在本研究中，在籼稻品种华粳县 74 (HJX74) 的遗传背景下，开发了 99 个携带南方稻供体片段的染色体单片段置换系 (SSSL)。 SSSL中99个取代片段的总长度为1580.16 cM，每个取代片段的平均长度为15.11 cM，覆盖了南方O.meridionalis基因组的873.94 cM和54.98%。对SSSLs的表型研究表明，3个SSSLs分别具有红色果皮、芒和种子落粒性，表明负责这些性状的南方稻的这些基因已转移到SSSLs中。 82个SSSL在抽穗期、产量相关性状等7个数量性状上存在较大变异。在P≤0.001时，77个SSSL在2014年秋季和2015年春季的至少一个性状上与HJX74相比，在至少一个性状上表现出显着差异，并且在24个SSSL中两季共检测到28个稳定QTL。这些结果表明，本研究建立的南方野生稻SSSLs文库为南方野生野生蝇有益基因的鉴定和转化提供了良好的种质平台，有利于南方野生野生蝇基因资源在体内的长期保存。
O. meridionalis is a wild species belonging to AA genome in the Oryza genus, which has a lot of beneficial genes for improvement of cultivated rice. In the present study, 99 chromosome single-segment substitution lines (SSSLs) were developed carrying donor segments of O. meridionalis in the genetic background of an indica cultivar, Huajingxian 74 (HJX74). The total lengths of the 99 substituted segments in the SSSLs were 1580.16 cM, with an average length of 15.11 cM per substituted segment, covering 873.94 cM and 54.98% of O.meridionalis genome. Phenotypic investigations of the SSSLs showed that three SSSLs had red pericarp, awn and showed seed shattering, respectively, indicating that these genes of O. meridionalis responsible for these traits have been transferred to the SSSLs. And wide variations were observed in seven quantitative traits including heading date and yield-related traits in 82 SSSLs.At P ≤ 0.001, 77 SSSLs showed significant differences compared with HJX74 in at least one trait either in the fall of 2014 or spring of 2015, and a total of 28 stable QTLs were detected in 24 SSSLs in both seasons. These results suggest that the SSSLs library of O. meridionalis developed in this study offers a good germplasm platform for the identification and transformation of beneficial genes of O. meridionalis, and facilitates the conservation of gene resources of O. meridionalis in vivo for long periods.