Effect of androstenedione on the growth and meiotic competence of bovine oocytes from early antral follicles
Effect of androstenedione on the growth and meiotic competence of bovine oocytes from early antral follicles
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DOI：
10.1017/s0967199411000268
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发表时间：
2012-11-01
期刊：
影响因子：
1.7
通讯作者：
Miyano, Takashi
中科院分区：
文献类型：
--
作者：
Taketsuru, Hiroaki;Hirao, Yuji;Miyano, Takashi
据报道，含有 17 β-雌二醇的培养基可支持从早期窦卵泡中分离出的牛卵母细胞的体外生长，直到最后阶段。本研究的目的是确定培养基中雄烯二酮对牛卵母细胞生长的影响。从早期窦卵泡中收集卵母细胞-颗粒细胞复合物，并在补充有 17 β-雌二醇（0、10 和 100 ng/ml）或雄烯二酮（0、10 和 100 ng/ml）的培养基中培养 14 天。接种到培养基上后测量的卵母细胞的平均直径为96.9μm（n=191）。任何一种类固醇对于在 14 天的培养期内维持卵母细胞-颗粒细胞复合物的组织都是必需的。在补充17β-雌二醇或雄烯二酮的培养基中，分别回收了约80％或65％的活卵母细胞。 14 天后，两组的卵母细胞大小均显着增加。将体外生长的卵母细胞进一步培养22-24小时以使卵母细胞成熟；在 10 ng/ml 和 100 ng/ml 17 β-雌二醇补充培养基中生长的卵母细胞分别有 13% 和 30% 达到中期 II；在补充雄烯二酮的培养基中生长的卵母细胞中超过 64% 成熟至中期 II。这些结果表明，雄烯二酮作为17β-雌二醇，可以维持牛卵母细胞-颗粒细胞复合物的活力并支持卵母细胞的生长，并且雄烯二酮在低剂量下有效促进卵母细胞减数分裂能力的获得。
Medium that contains 17 beta-estradiol has been reported to support in vitro growth of bovine oocytes, isolated from early antral follicles, until the final stage. The aim of this study was to determine the effects of androstenedione in medium on such growing bovine oocytes. Oocyte-granulosa cell complexes were collected from early antral follicles and cultured for 14 days in medium supplemented with 17 beta-estradiol (0, 10 and 100 ng/ml) or androstenedione (0, 10 and 100 ng/ml). The mean diameter of oocytes measured after seeding on the culture substrate was 96.9 mu m (n = 191). Either steroid was necessary for maintainance of the organization of oocyte-granulosa cell complexes over the 14-day culture period. In the 17 beta-estradiol- or the androstenedione-supplemented medium about 80% or 65%, respectively, of viable oocytes were recovered. In both groups the increase in oocyte size was significant after 14 days. The in vitro grown oocytes were cultured for a further 22-24 h for oocyte maturation; 13% and 30% of oocytes grown in the 10 and 100 ng/ml 17 beta-estradiol-supplemented medium reached metaphase II, respectively; more than 64% of oocytes grown in the androstenedione-supplemented medium matured to metaphase II. These results show that androstenedione, as 17 beta-estradiol, can maintain the viability of bovine oocyte-granulosa cell complexes and support the growth of oocytes, and that androstenedione promotes the acquisition of oocyte meiotic competence efficiently at a low dose.