ESTRADIOL, TESTOSTERONE, AND ANDROSTENEDIONE IN OVINE FOLLICULAR-FLUID DURING GROWTH AND ATRESIA OF OVARIAN FOLLICLES
ESTRADIOL, TESTOSTERONE, AND ANDROSTENEDIONE IN OVINE FOLLICULAR-FLUID DURING GROWTH AND ATRESIA OF OVARIAN FOLLICLES
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DOI：
10.1095/biolreprod24.1.105
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发表时间：
1981-01-01
影响因子：
3.6
通讯作者：
BURGER, HG
中科院分区：
文献类型：
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作者：
CARSON, RS;FINDLAY, JK;BURGER, HG
雌二醇-17的测定。从已知直径和形态闭锁阶段的窦卵泡组中获得的卵泡液中睾酮和雄烯二酮表明，在非闭锁生长过程中甾体生成有两个阶段。在从直径1-3毫米的卵泡中获得的液体池中，在腔形成后不久，睾酮浓度短暂增加4倍，为99-420 nM，而雄烯二酮和雌二醇-17 - β的浓度则增加4倍。分别为4 ~ 16 nM和10 ~ 47 nM。在直径为30mm的非闭锁卵泡池液中，雌二醇-17。超过睾酮和雄烯二酮，随着卵泡直径的增加，从9 nM增加到341 nM。睾酮浓度从247 nM逐渐下降到56 nM，雄烯二酮浓度随着卵泡直径超过3 mm而从22 nM下降到7 nM。早期闭锁卵泡液中3种甾体激素浓度与非闭锁卵泡无显著差异，但闭锁后期睾酮浓度升高，雌二醇浓度降低。从单个卵泡中获得的数据证实了最初的发现，即卵泡液池中雌激素与总卵泡雄激素比率的降低与各种大小的卵泡闭锁有关。伴随闭锁的甾体生成模式的变化可能反映了卵泡在闭锁过程中功能能力的一些早期变化，但不一定是闭锁的主要原因。
Measurement of estradiol-17.beta., testosterone and androstenedione in follicular fluid obtained from groups of antral follicles of known diameter and stage of morphological atresia indicates 2 phases of steroidogenesis during nonatretic growth. In pools of fluid obtained from follicles of 1-3 mm diameter, a transient 4-fold increase in testosterone concentrations of 99-420 nM was observed soon after antrum formation, whereas concentrations of androstenedione and estradiol-17.beta. ranged 4-16 and 10-47 nM, respectively. In the pooled fluid of nonatretic follicles of > 3 mm diameter, concentrations of estradiol-17.beta. exceeded that of either testosterone or androstenedione, and increased from 9 to 341 nM as follicular diameter increased. Testosterone concentrations progressively decreased from 247 to 56 nM, and the concentration of androstenedione decreased from 22 to 7 nM as follicular diameter increased beyond 3 mm. Concentrations of all 3 steroids in pools of fluid from early atretic follicles were not significantly different from those of nonatretic follicles, but in the later stages of atresia concentrations of testosterone remained elevated and those of estradiol stayed low at all stages of growth. Data obtained from individual follicles confirmed the initial finding that a reduction in the ratio of estrogen to total follicular androgen in pools of follicular fluid was associated with atresia of all sizes of follicles. The changes in patterns of steroidogenesis which accompany atresia may reflect some of the earliest changes in the functional capacity of follicles undergoing atresia, but are not necessarily the primary cause of atresia.