Transcription analysis using high-density micro-arrays of Aspergillus nidulans wild-type and creA mutant during growth on glucose or ethanol
Transcription analysis using high-density micro-arrays of Aspergillus nidulans wild-type and creA mutant during growth on glucose or ethanol
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DOI：
10.1016/j.fgb.2006.03.003
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发表时间：
2006-08-01
影响因子：
3
通讯作者：
Nielsen, Jens
中科院分区：
文献类型：
--
作者：
Mogensen, Jesper;Nielsen, H. Bjorn;Nielsen, Jens
在这里，我们描述了最近公布的构巢拟谷盗基因组序列[Galagan，JE.，卡尔沃，东南，科莫角，Li-Jun，M.，Wortman，Jr.，例如，2005.构巢曲霉基因序列测定及与A.烟曲霉A.米。Nature 438（7071），1105-1115]使用Febit Geniom One阵列系统设计具有3278个选择的基因的探针的高密度寡核苷酸阵列。为此，使用程序OligoWiz II设计24，125个探针以覆盖3278个选择的基因。随后，使用Febit系统通过比较creA缺失突变株与以葡萄糖或乙醇作为唯一碳源生长的参考株的基因表达来研究碳分解代谢物阻遏。为了鉴定共调节基因和受碳源或CreA影响的基因，最显著调节的基因（p
Here, we describe how the recently published Aspergilhis nidulans genome sequence [Galagan, JE., Calvo, S.E., Cuomo, C., Li-Jun, M., Wortman, JR., et al., 2005. Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature 438 (7071), 1105-1115] was used to design a high-density oligo array with probes for 3278 selected genes using the Febit Geniom((R)) One array system. For this purpose, the program OligoWiz II was used to design 24,125 probes to cover the 3278 selected genes. Subsequently, the Febit system was used to investigate carbon catabolite repression by comparing the gene expression of a creA deleted mutant strain with a reference strain grown either with glucose or ethanol as the sole carbon source. In order to identify co-regulated genes and genes influenced by either the carbon source or CreA, the most significantly regulated genes (p