Vertically aligned carbon nanofiber electrode arrays for nucleic acid detection
Vertically aligned carbon nanofiber electrode arrays for nucleic acid detection
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DOI：
10.1016/j.cplett.2010.09.048
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发表时间：
2010-10-29
影响因子：
2.8
通讯作者：
Meyyappan, M.
中科院分区：
文献类型：
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作者：
Arumugam, Prabhu U.;Yu, Edmond;Meyyappan, M.
我们提出了使用由垂直排列的碳纳米纤维(VACNF)电极组成的纳米电极阵列来电化学检测对应于大肠杆菌O157：H7 16S rRNA基因的DNA靶标。在电极结构中，采用对二甲苯C作为填充“基质”的材料，以避免高温加工。这种易于沉积的几微米高的薄膜在高深宽比的VACNF之间提供了一层共形涂层，而针孔可以忽略不计。低本底电流显示了该方法用于超灵敏检测的潜力。使用简单的电极制备可获得一致且可重现的电化学信号。这种简单、可靠、低成本的方法是为病原体检测、早期癌症诊断和环境监测等应用开发实用传感器的前进一步。(C)2010爱思唯尔B.V.保留所有权利。
We present electrochemical detection of DNA targets that corresponds to Escherichia coli O157:H7 16S rRNA gene using a nanoelectrode array consisting of vertically aligned carbon nanofiber (VACNF) electrodes. Parylene C is used as gap filling 'matrix' material to avoid high temperature processing in electrode construction. This easy to deposit film of several micron heights provides a conformal coating between the high aspect ratio VACNFs with negligible pin-holes. The low background currents show the potential of this approach for ultra-sensitive detection. Consistent and reproducible electrochemical-signals are achieved using a simple electrode preparation. This simple, reliable and low-cost approach is a forward step in developing practical sensors for applications like pathogen detection, early cancer diagnosis and environmental monitoring. (C) 2010 Elsevier B.V. All rights reserved.