Photodynamic Therapy Associated with Conventional Endodontic Treatment in Patients with Antibiotic-resistant Microflora: A Preliminary Report
Photodynamic Therapy Associated with Conventional Endodontic Treatment in Patients with Antibiotic-resistant Microflora: A Preliminary Report
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DOI：
10.1016/j.joen.2010.06.001
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发表时间：
2010-09-01
影响因子：
4.2
通讯作者：
Ribeiro, Martha S.
中科院分区：
文献类型：
--
作者：
Garcez, Aguinaldo S.;Nunez, Silvia C.;Ribeiro, Martha S.
前言：本研究报告了光动力疗法(PDT)联合根管治疗对先前抗生素治疗耐药的牙髓坏死菌感染患者的抗菌效果。方法：选择经常规根管治疗和抗生素治疗的21例根尖周病变患者的30颗前牙。在进入根管后，(2)在根管治疗后，(3)在光动力疗法后，采集微生物样本。结果：所有患者至少有1株细菌对抗生素耐药。PDT以聚乙烯亚胺(E6)为光敏剂，半导体激光器为光源(P=40 mW，t=4min，E=9.6J)。单纯根管治疗可显著减少细菌种类，但只有3颗牙无菌，而联合应用牙髓治疗和光动力疗法可消除所有耐药菌，所有牙齿无菌。结论：在常规根管治疗的基础上加用光动力疗法，可进一步显著降低微生物负荷。光动力疗法是杀灭耐多药微生物的有效方法。(J Endod 2010；36：1463-1466)
Introduction: This study reports the antimicrobial effect of photodynamic therapy (PDT) combined with endodontic treatment in patients with necrotic pulp infected with microflora resistant to a previous antibiotic therapy. Methods: Thirty anterior teeth from 21 patients with periapical lesions that had been treated with conventional endodontic treatment and antibiotic therapy were selected. Microbiological samples were taken (1) after accessing the root canal, (2) after endodontic therapy, and (3) after PDT. Results: All the patients had at least 1 microorganism resistant to antibiotics. PDT used polyethylenimine chlorin(e6) as a photosensitizer and a diode laser as a light source (P = 40 mW, t = 4minutes, E = 9.6 J). Endodontic therapy alone produced a significant reduction in numbers of microbial species but only 3 teeth were free of bacteria, whereas the combination of endodontic therapy with PDT eliminated all drug-resistant species and all teeth were bacteria-free. Conclusions: The use of PDT added to conventional endodontic treatment leads to a further major reduction of microbial load. PDT is an efficient treatment to kill multi-drug resistant microorganisms. (J Endod 2010;36:1463-1466)