The structure of anammox granular sludge under varying long-term organic matter stress: Performance, physiochemical and microbial community
The structure of anammox granular sludge under varying long-term organic matter stress: Performance, physiochemical and microbial community
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不同长期有机物胁迫下厌氧氨氧化颗粒污泥的结构：性能、理化和微生物群落
DOI：
10.1016/j.jclepro.2021.129117
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发表时间：
2021-09-30
影响因子：
11.1
通讯作者：
Xie, Jiawei
中科院分区：
文献类型：
--
作者：
Chen, Chongjun;Jiang, Ying;Xie, Jiawei
在UASB（上流式厌氧污泥床/毯）反应器中，研究了不同浓度有机物对厌氧氨氧化颗粒污泥脱氮、理化特性和微生物群落的长期应激影响。随着化学需氧量（COD）浓度从0 mg L-1增加到200 mg L-1，氨氮去除率从97.71%下降到46.52%，亚硝酸盐去除率保持在96.78 ~ 98.62%。COD投加量为50 mg L-1时，TN去除率最高，达到98.00%。150mg L-1和200mg L-1的COD会引起厌氧氨氧化颗粒污泥的崩解，同时也会降低胞外聚合物（EPS）含量、机械强度和沉降性能。根据高通量Miseq测序分析，门水平上的主要细菌已经从Chloroflexi转移到Proteobacteria。在长期有机物胁迫下，候选菌Brocadia成为厌氧氨氧化菌的优势菌属，与氨氮和总氮去除效率、不稳定系数（IC）、蛋白质/多糖（PN/PS）和粒径呈正相关，与COD浓度和EPS呈负相关。
Long-term stress effects of varying concentrations of organic matter on nitrogen removal, physiochemical characteristics and microbial community of anammox granular sludge were evaluated in UASB (Up-flow Anaerobic Sludge Bed/Blanket) reactors. With increase of COD (Chemical Oxygen Demand) concentration (made by glucose) from 0 mg L-1 to 200 mg L-1, ammonium removal efficiency decreased sequentially from 97.71% to 46.52%, while the nitrite removal efficiency was maintained at 96.78-98.62%. The TN removal efficiency reached the highest (98.00%) at 50 mg L-1 of COD. However, 150 and 200 mg L-1 of COD caused the disintegration of anammox granular sludge, while also reducing extracellular polymeric substances (EPS) content, mechanical strength and sedimentation performance, respectively. The main bacteria at the phylum level had shifted from Chloroflexi to Proteobacteria, according to high-throughput Miseq sequencing analyses. Candidatus Brocadia became the dominant anammox genus under long-term organic matter stress, which correlated positively with ammonium and TN removal efficiency, instability coefficient (IC), protein/polysaccharide (PN/PS) and particle size, but negatively with COD concentration and EPS, respectively.