Stress, geomagnetic disturbance, infradian and circadian sampling for circulating corticosterone and models of human depression?
Stress, geomagnetic disturbance, infradian and circadian sampling for circulating corticosterone and models of human depression?
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DOI：
10.1007/bf03033560
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发表时间：
2008-04-01
影响因子：
3.7
通讯作者：
Halberg, F.
中科院分区：
文献类型：
--
作者：
Olah, A.;Jozsa, R.;Halberg, F.
虽然某些昼夜荷尔蒙变化是显著的，但其可预测的评估需要标准化的采样条件。自20世纪50年代以来，啮齿动物循环皮质酮的24小时节律被研究，作为假定的压力代理，108只老鼠被分成9组，每组6只雄性动物和9组6只雌性动物，每4小时采样一次，持续24小时。在第一次压力研究中，在两个对照组和18个被考虑组中的16个组中，“无节奏”（零振幅）假设未能在5%的概率水平上被拒绝。然而，在同一实验室进行的三项独立的后续研究中，168只大鼠分别接受两种反相照明方案，每4小时采样7天或14天，可以检测到具有统计学意义的昼夜节律。在第一项应激研究中，将特定组集中在一起有助于检测和评估昼夜皮质酮节律。除了皮质酮以外，其他激素可能变化更慢或调整不同，以响应不同的同步器，三个后续研究产生了不确定性测量（95%置信区间），用于其昼夜节律周期的点估计，可能在任何未来的研究中用作参考标准。在两项后续研究开始时偶然出现的磁干扰与检测到的一种环casemiseptan成分有关，这就提出了一个问题，即地磁干扰是否可以被视为一种“负载”。远远超出了样本量的限制，在抑郁症模型的背景下考虑了实验实验室中关于研究设计的标准化方法要求。报告指出了自然界不可预见的地磁贡献和人类研究的教训，所有这些都是为了支持在负荷研究中倡导超过24小时的采样时间表。
While certain cireadian hormonal changes are prominent, their predictable assessment requires a standardization of conditions of sampling. The 24-hour rhythm in circulating corticosterone of rodents, known since the 1950s, was studied as a presumed proxy for stress on 108 rats divided into 9 groups of 6 male and 9 groups of 6 female animals sampled every 4 hours for 24 hours. In a first stress study, the "no-rhythm" (zero-amplitude) assumption failed to be rejected at the 5% probability level in the two control groups and in 16 out of the 18 groups considered. A circadian rhythm could be detected with statistical significance, however, in three separate follow-up studies in the same laboratory, each on 168 rats kept on two antiphasic lighting regimens, with 4-hourly sampling for 7 or 14 days. In the first stress study, pooling of certain groups helped the detection and assessment of the circadian corticosterone rhythm. Without extrapolating to hormones other than corticosterone, which may shift more slowly or adjust differently and in response to different synchronizers, the three follow-up studies yielded uncertainty measures (95% confidence intervals) for the point estimate of its circadian period, of possible use in any future study as a reference standard.The happenstance of a magnetic disturbance at the start of two follow-up studies was associated with the detection of a circasemiseptan component, raising the question whether a geomagnetic disturbance could be considered as a "load". Far beyond the limitations of sample size, the methodological requirements for standardization in the experimental laboratory concerning designs of studies are considered in the context of models of depression. Lessons from nature's unforeseen geomagnetic contribution and from human studies are noted, all to support the advocacy, in the study of loads, of sampling schedules covering more than 24 hours.