Food deprivation increases the expression of melanocortin-4 receptor in the liver of barfin flounder, Verasper moseri
Food deprivation increases the expression of melanocortin-4 receptor in the liver of barfin flounder, Verasper moseri
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DOI：
10.1016/j.ygcen.2007.05.010
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发表时间：
2008-01-15
影响因子：
2.7
通讯作者：
Takahashi, Akiyoshi
中科院分区：
文献类型：
--
作者：
Kobayashi, Yuki;Tsuchlya, Keisuke;Takahashi, Akiyoshi
黑素皮质素（melanocortin，MC）系统由促黑素细胞激素（melanocyto-stimulatinghormone，MT）、促肾上腺皮质激素（adrenocorticotropichormone，AR）及其受体组成。MC系统在色素沉着和能量稳态调节中均起作用，其中MC 4 R作为五种MC受体之一具有关键作用。有趣的是，barfin比目鱼（Pleuronectiformes）饲养的黑色背景显示出迟缓的增长相比，白色背景饲养的鱼，这可能与MC系统，因为它的双重作用，在调节色素沉着和能量状态。在这里，我们克隆MC 4 R和评估的影响，其表达的barfin比目鱼的摄食状态。Barfin flounder MC4R.序列分析表明，该基因由325个氨基酸组成，与河豚鱼MC 4 R的同源性最高（85%），其次为虹鳟鱼（82%）、斑马鱼（79%）、金鱼（78%）、角鲨（71%）、鸡（67%）、人（67%）和小鼠（65%）。在检测的18种不同组织中，用反转录PCR检测到MC 4 R在脑、肝、睾丸和卵巢中的优势表达。食物剥夺导致肝脏中MC 4 R转录本数量增加4倍，而在禁食鱼和喂食对照组之间的大脑中没有观察到变化。这些结果表明，NIC系统，包括MC 4 R与能量稳态的barfin比目鱼和外周组织可能在这种调节中发挥作用。(c)2007爱思唯尔公司All rights reserved.
The melanocortin (MC) system is composed of melanocyte-stimulating hormone, adrenocorticotropic hormone and their receptors. The MC system has a role in both pigmentation and the regulation of energy homeostasis, in which MC4R, one of the five MC receptors, has a key role. Interestingly, the barfin flounder (Pleuronectiformes) reared with a black background shows retarded growth compared to white background-reared fish, which could be associated with the MC system because of its dual role in regulating pigmentation and energy status. Here, we cloned MC4R and assessed the effects of feeding status on its expression in barfin flounder. Barfin flounder MC4R. was composed of 325 amino acids and showed the highest sequence identity to MC4R of fugu (85%), followed by rainbow trout (82%), zebrafish (79%), goldfish (78%), dogfish (71%), chickens (67%), humans (67%) and mice (65%). Among 18 different tissues examined, the predominant expression of MC4R was observed in the brain, liver, testis and ovary as detected with reverse transcription PCR. Food deprivation resulted in a 4-fold increase in the number of MC4R transcripts in the liver, whereas no change was observed in the brain between fasted fish and fed controls. These results suggest that the NIC system including MC4R is associated with energy homeostasis in barfin flounder and that peripheral tissues could play a role in this regulation. (c) 2007 Elsevier Inc. All rights reserved.