Enniatin Production Influences Fusarium avenaceum Virulence on Potato Tubers, but not on Durum Wheat or Peas
Enniatin Production Influences Fusarium avenaceum Virulence on Potato Tubers, but not on Durum Wheat or Peas
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DOI：
10.3390/pathogens9020075
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发表时间：
2020-02-01
期刊：
影响因子：
3.7
通讯作者：
Foroud, Nora A.
中科院分区：
文献类型：
--
作者：
Eranthodi, Anas;Schneiderman, Danielle;Foroud, Nora A.
燕麦镰刀菌（Fusarium avenaceum）是引起许多作物物种病害的一种多面手病原体。该真菌产生一系列真菌毒素，包括环己缩酚肽恩镰孢菌素。真菌毒素是植物与病原菌相互作用中的致病因子和毒力因子，恩镰孢菌素对马铃薯块茎的侵袭性有影响。为了确定这些真菌毒素在其他真菌中的作用。燕麦-宿主相互作用，产生了ENNIATIN SYNTHASE 1（ESYN 1）破坏和过表达突变体，并研究了它们感染小麦和豌豆的能力。作为初步研究，筛选转化体引起马铃薯块茎坏死的能力，并且与先前的报道一致，恩镰孢菌素的产生增加了块茎上的坏死病变大小。相比之下，当评估相同的突变体在豌豆根或硬粒小麦穗中引起疾病的能力时，没有观察到疾病症状或毒性的变化。虽然已知，至少在小麦的情况下，外源施用恩镰孢菌素可引起组织坏死，但这组真菌毒素本身似乎不是豌豆或硬粒小麦疾病发展的关键因素。
Fusarium avenaceum is a generalist pathogen responsible for diseases in numerous crop species. The fungus produces a series of mycotoxins including the cyclohexadepsipeptide enniatins. Mycotoxins can be pathogenicity and virulence factors in various plant-pathogen interactions, and enniatins have been shown to influence aggressiveness on potato tubers. To determine the role of these mycotoxins in other F. avenaceum-host interactions, ENNIATIN SYNTHASE 1 (ESYN1) disruption and overexpression mutants were generated and their ability to infect wheat and peas investigated. As a preliminary study, the transformants were screened for their ability to cause potato tuber necrosis and, consistent with a previous report, enniatin production increased necrotic lesion size on the tubers. By contrast, when the same mutants were assessed in their ability to cause disease in pea roots or durum wheat spikes, no changes in disease symptoms or virulence were observed. While it is known that, at least in the case of wheat, exogenously applied enniatins can cause tissue necrosis, this group of mycotoxins does not appear to be a key factor on its own in disease development on peas or durum wheat.