Predictive value of morphologically normal sperm concentration in the medium for in-vitro fertilization.
Predictive value of morphologically normal sperm concentration in the medium for in-vitro fertilization.
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体外受精培养基中形态正常精子浓度的预测价值。
DOI：
10.1111/j.1365-2605.1993.tb01163.x
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发表时间：
1993
影响因子：
--
通讯作者：
J. Geraedts
中科院分区：
文献类型：
--
作者：
M. Enginsu;J. Dumoulin;M. Pieters;J. Evers;J. Geraedts
采用严格标准（MEUSC）和常规精子参数进行精子形态学评价，研究上游选择程序前后的体外受精和妊娠结局。将50，000个进行性活动精子与解冻的卵母细胞授精，本研究评估了授精样本中精子总数和严格正常形态的百分比对IVF结果的影响。结果表明，无论是在自然还是在游泳后样本中，使用严格的标准，具有正常形态的精子的百分比是IVF结果的最佳预测因子。他们的分界点分别为5%和8%。受精后形态正常的精子数与受精率也有很好的相关性。然而，不可能找到该参数的适当截止点。根据患者的原生和游泳后评分对患者进行分类。第1类，其中两个参数均低于其各自的临界点，显示7%的受精率和0%的妊娠率。第3类，其中两个参数均高于其临界点，显示70%的受精率和23%的妊娠率。这表明精子形态可以作为IVF患者选择的标准，以帮助识别可能的生育力低下的男性。
Morphological evaluation of spermatozoa using strict criteria (MEUSC) and conventional sperm parameters were studied with respect to in-vitro fertilization and pregnancy outcome before and after a swim-up selection procedure. Recovered oocytes were inseminated with 50,000 progressively motile spermatozoa, and this study assess the influence of the total number of spermatozoa and of the percentage with strictly normal morphology in the insemination sample on the outcome of IVF. The results showed that the percentages of spermatozoa with normal morphology using strict criteria, both in native and in post-swim-up samples, were the best predictors of IVF outcome. Their respective cut-off points were 5% and 8%. The number of morphologically normal spermatozoa inseminated also showed a good correlation with fertilization. However, it was not possible to find a proper cut-off point for this parameter. The patients were categorized on the basis of their native and post-swim-up scores. Category 1, in which both parameters were below their respective cut-off points, showed a 7% fertilization rate and a 0% pregnancy rate. Category 3, in which both parameters were above their cut-off points, showed a 70% fertilization rate and a 23% pregnancy rate. This suggests that sperm morphology can be used as a criterion for patient selection for IVF as an aid to identification of possibly subfertile males.