Identification of a novel pathogen-induced gene encoding a leucine-rich repeat protein expressed in phloem cells of Capsicum annuum
Identification of a novel pathogen-induced gene encoding a leucine-rich repeat protein expressed in phloem cells of Capsicum annuum
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DOI：
10.1016/s0167-4781(03)00120-9
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发表时间：
2004-02-20
期刊：
影响因子：
--
通讯作者：
Hwang, BK
中科院分区：
文献类型：
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作者：
Jung, EH;Jung, HW;Hwang, BK
CALRR1 基因在被黄单胞菌 pv 感染后在辣椒叶中表达。 vesicatoria，编码一个分泌性富含亮氨酸重复序列 (LRR)，具有 24 个氨基酸 LRR 基序的 5 个串联重复序列 Northern 印迹分析表明，CALRR1 在辣椒植物中不是组成型表达，但在 X Campestris pv. 感染后被强烈诱导表达。叶上的水泡菌、辣椒疫霉、Colletotrichum coccodes 和 Colletotrichum gloeosporioides。 CALRR1 不是通过细菌感染在上部叶中系统诱导的。细菌活细胞的接种以及死细胞和病原性或非病原性细菌培养滤液的处理引发了 CALRR1 转录本的积累。用信号分子（包括水杨酸（SA）、乙烯（ET）、茉莉酸甲酯（MeJA）、DL-β-氨基正丁酸（BABA）和苯并噻二唑（BTH））处理不会激活CALRR1基因的转录，表明CALRR1表达不受这些分子激活的防御信号通路的调节。 CALRR1 是由高盐度、脱落酸 (ABA) 和创伤处理诱导的，但不是由干旱和寒冷胁迫诱导的。原位杂交研究表明，在病原体感染和ABA暴露期间，CALRR1 mRNA定位于辣椒植物叶、茎和绿色果实的韧皮部组织中。 CALRR1的位置特征和时空表达模式表明它可能在保护韧皮部细胞免受影响韧皮部功能的生物和非生物胁迫中发挥作用。 (C) 2004 Elsevier B.V. 保留所有权利。
The CALRR1 gene, expressed in pepper leaves following infection by Xanthomonas campestris pv. vesicatoria, encodes a secreted leucine-rich repeat (LRR) with five tandem repeats of a 24-amino-acid LRR motif Northern blot analyses revealed that CALRR1 is not constitutively expressed in pepper plants, but is strongly induced upon the infection by X campestris pv. vesicatoria, Phytophthora capsici, Colletotrichum coccodes and Colletotrichum gloeosporioides on leaves. CALRR1 was not systemically induced in upper leaves by bacterial infection. The inoculation of bacterial live cells, and treatment with dead cells and culture filtrates of pathogenic or nonpathogenic bacteria triggered the accumulation of CALRR1 transcripts. Treatment with signaling molecules, including sallicylic acid (SA), ethylene (ET), methyl jasmonate (MeJA), DL-beta-amino-n-butyric acid (BABA) and benzothiadiazole (BTH), did not activate the transcription of the CALRR1 gene, indicating that CALRR1 expression is not regulated by defense signaling pathways activated by these molecules. CALRR1 was induced by treatment with high salinity, abscisic acid (ABA) and wounding, but not by drought and cold stress. An in situ hybridization study showed that CALRR1 mRNA was localized in phloem tissues of leaves, stems, and green fruits of pepper plants during the pathogen infection and ABA exposure. The location characteristics and the spatio-temporal expression pattern of CALRR1 suggest that it may play a role in protecting phloem cells against biotic and abiotic stresses affecting phloem function. (C) 2004 Elsevier B.V. All rights reserved.