Role of ARF4L in recycling between endosomes and the plasma membrane
Role of ARF4L in recycling between endosomes and the plasma membrane
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DOI：
10.1023/b:cemn.0000012719.12015.ec
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发表时间：
2004-02-01
影响因子：
4
通讯作者：
Tohyama, M
中科院分区：
文献类型：
--
作者：
Katayama, T;Imaizumi, K;Tohyama, M
ARF4L是人类ADP核糖化因子样蛋白，是ARF家族的成员，是一种小的GTP结合蛋白，在囊泡运输和蛋白质分泌中发挥重要作用。然而，对ARF4L的生理作用知之甚少。在本研究中，为了了解ARF4L的生物学功能，我们对功能区突变的ARF4L分子进行了免疫细胞化学分析。ARF4L在与GTP结合后分布于质膜(Q80L)，在与GDP结合后分布于内体(T35N)。此外，非活性形式的ARF4L(T35N)导致转铁蛋白受体定位于内膜室，而活性形式(Q80L)则导致转铁蛋白受体转运至质膜。这些发现表明，ARF4L驱动货物蛋白的运输，以及随后回收小泡与质膜的融合，以维持细胞表面。
The human ADP-ribosylation factor-like protein, ARF4L is a member of the ARF family, which are small GTP-binding proteins that play significant roles in vesicle transport and protein secretion. However, little is known about the physiological roles of ARF4L. In this study, to understand the biological functions of ARF4L, we carried out immunocytochemical analysis of ARF4L molecules with mutations in the functional domains. ARF4L was shown to be distributed to the plasma membrane following binding to GTP (Q80L), and into endosomes following binding to GDP (T35N). Moreover, the inactive-form of ARF4L ( T35N) causes localization of transferrin receptors to the endosomal compartment, while the active form ( Q80L) causes transport to the plasma membrane. These findings indicate that ARF4L drive the transport of cargo protein and subsequent fusion of recycling vesicles with the plasma membrane for maintenance of the cell surface.