DIFFERENT T-HELPER CELL SUBSETS ELICITED IN MICE UTILIZING 2 DIFFERENT ADJUVANT VEHICLES - THE ROLE OF ENDOGENOUS INTERLEUKIN-1 IN PROLIFERATIVE RESPONSES
DIFFERENT T-HELPER CELL SUBSETS ELICITED IN MICE UTILIZING 2 DIFFERENT ADJUVANT VEHICLES - THE ROLE OF ENDOGENOUS INTERLEUKIN-1 IN PROLIFERATIVE RESPONSES
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DOI：
10.1016/0008-8749(89)90011-7
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发表时间：
1989-06-01
影响因子：
4.3
通讯作者：
MAURER, PH
中科院分区：
文献类型：
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作者：
GRUN, JL;MAURER, PH
用两种不同佐剂制剂（明矾 (AlK(SO4)2) 或完全弗氏佐剂 (CFA)）中含有的聚（Glu、Arg、Ala）或聚（Glu、Lys、Phe）对小鼠进行免疫，4-16 天后测定体外抗原驱动的淋巴结细胞增殖反应。用明矾上的抗原进行免疫后，内源性白细胞介素 1 (IL-1) 以及白细胞介素 4 (IL-4) 的产生对于所引发的 T 细胞的增殖是必需的，因为包含多克隆山羊抗小鼠 IL-1.alpha。或培养物中的单克隆抗小鼠 IL-4 (11b11) 抑制对抗原的增殖反应。相反，CFA中抗原引发的细胞增殖反应不受抗IL-1或抗IL-4的抑制。无论使用哪种佐剂来引发抗原反应性细胞，单克隆抗小鼠 CD4 (GK1.5) 都会抑制增殖反应。这些数据表明，表型不同的CD4+细胞亚群是由在不同佐剂制剂中施用的相同抗原引发的，用明矾上的聚合物免疫后产生Th2样细胞，用CFA中的抗原免疫后产生Th1样细胞。对免疫小鼠血清中存在的聚合物特异性抗体同种型的检查也支持了这一结论。
Mice were immunized with either poly(Glu, Arg, Ala) or poly(Glu, Lys, Phe) contained in two different adjuvant preparations, alum (AlK(SO4)2) or complete Freund''s adjuvant (CFA), and in vitro antigen-driven proliferative responses fo lymph node cells were assayed 4-16 days later. After immunization with antigens on alum, endogenous interleukin 1 (IL-1) as well as interleukin 4 (IL-4) production was required for the proliferation of the elicited T cells as inclusion of either polyclonal goat anti-mouse IL-1.alpha. or monoclonal anti-mouse IL-4 (11b11) in the cultures inhibited proliferative responses to antigen. In contrast, proliferative responses of cells elicited by antigen in CFA were not inhibited by either anti-IL-1 or anti-IL-4. Monoclonal anti-mouse CD4 (GK1.5) inhibited proliferative responses regardless of which adjuvant was used to elicit antigen-reactive cells. These data indicated that phenotypically different subpopulations of CD4+ cells were elicited by the same antigen administered in different adjuvant preparations, Th2-like cells after immunization with polymers on alum and Th1-like cells after immunization with antigens in CFA. An examination of the isotypes of polymer-specific antibodies present in the sera of immunized mice also supported this conclusion.