State of Water, Molecular Structure, and Cytotoxicity of Silk Hydrogels
State of Water, Molecular Structure, and Cytotoxicity of Silk Hydrogels
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DOI：
10.1021/bm200221u
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发表时间：
2011-06-01
影响因子：
6.2
通讯作者：
Sakai, Takamasa
中科院分区：
文献类型：
--
作者：
Numata, Keiji;Katashima, Takuya;Sakai, Takamasa
发展了一种通过调节丝蛋白浓度来调节丝蛋白水凝胶体相含水量的新技术，这有助于研究水凝胶中水的状态对其细胞毒性等生物学功能的影响。用乙醇诱导丝素水凝胶的凝胶化，并通过流变仪分析其凝胶化行为。在各种丝浓度制备的丝水凝胶的特征在于相对于它们的水含量，分子和网络结构，水的状态，机械性能，和细胞毒性的人间充质干细胞。丝素水凝胶的网络结构是不均匀的，具有β-折叠和原纤结构。通过差示扫描量热法和细胞增殖实验研究了丝素水凝胶中水的状态对细胞毒性的影响，结果表明，结合水可以支持细胞基质中的细胞粘附蛋白与丝素水凝胶表面相互作用。
A novel technique was developed to regulate the bulk water content of silk hydrogels by adjusting the concentrations of silk proteins, which is helpful to investigate the effects of the state of water in polymeric hydrogel on its biological functions, such as cytotoxicity. Gelation of the silk hydrogel was induced with ethanol and its gelation behavior was analyzed by rheometry. The silk hydrogels prepared at various silk concentrations were characterized with respect to their water content, molecular and network structures, state of water, mechanical properties, and cytotoxicity to human mesenchymal stem cells. The network structure of silk hydrogel was heterogeneous with beta-sheet and fibrillar structures. The influence of the state of water in the silk hydrogel on the cytotoxicity was recognized by means of differential scanning calorimetry and cell proliferation assay, which revealed that the bound water will support cell-adhesion proteins in the cellular matrix to interact with the surface of the silk hydrogels.