Lack of delta waves and sleep disturbances during non-rapid eye movement sleep in mice lacking α1G-subunit of T-type calcium channels
Lack of delta waves and sleep disturbances during non-rapid eye movement sleep in mice lacking α1G-subunit of T-type calcium channels
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DOI：
10.1073/pnas.0408089101
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发表时间：
2004-12-28
影响因子：
11.1
通讯作者：
Shin, HS
中科院分区：
文献类型：
--
作者：
Lee, J;Kim, D;Shin, HS
T型钙通道被认为是睡眠期间脑节律的起搏器，但其对睡眠行为状态的贡献相对不确定。在这里，我们发现，缺乏α 1（G）T型Ca 2+通道的小鼠表现出丘脑δ波（1-4 Hz）的损失和睡眠纺锤波（7- 1-4 Hz）的减少，而缓慢（< 1 Hz）的节奏相对完整，与野生型相比，在氨基甲酸乙酯麻醉和非快速眼动（NREM）睡眠期间。对睡眠障碍的分析（定义为NREM睡眠期间短暂觉醒（BA）事件的发生）显示，与野生型相比，突变小鼠表现出更高的> 16秒BA的发生率，而两种基因型之间的BA < 16秒没有差异。这些结果与之前的想法一致，即δ振荡和睡眠纺锤波的不同性质来自皮质产生的慢波。这些结果也表明，α 1（G）-亚单位的T-型钙通道在丘脑皮层振荡的发生中起着关键作用，并有助于睡眠状态的调制和NREM睡眠和觉醒状态之间的过渡。
T-type calcium channels have been implicated as a pacemaker for brain rhythms during sleep but their contribution to behavioral states of sleep has been relatively uncertain. Here, we found that mice lacking alpha1(G) T-type Ca2+ channels showed a loss of the thalamic delta (1-4 Hz) waves and a reduction of sleep spindles (7-14 Hz), whereas slow (< 1 Hz) rhythms were relatively intact, when compared with the wild-type during urethane anesthesia and non-rapid eye movement (NREM) sleep. Analysis of sleep disturbances, as defined by the occurrence of brief awakening (BA) episodes during NREM sleep, revealed that mutant mice exhibited a higher incidence of BAs of > 16 sec compared with the wild-type, whereas no difference was seen in BAs of < 16 sec between the two genotypes. These results are consistent with the previous idea of the distinct nature of delta oscillations and sleep spindles from cortically generated slow waves. These results also suggest that the alpha1(G)-subunit of T-type calcium channels plays a critical role in the genesis of thalamocortical oscillations and contributes to the modulation of sleep states and the transition between NREM sleep and wake states.