Organoid culture of human prostate cancer cell lines LNCaP and C4-2B.
Organoid culture of human prostate cancer cell lines LNCaP and C4-2B.
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DOI：
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发表时间：
2017
影响因子：
1.2
通讯作者：
Lin Ma;Jingwu Li;Q. Nie;Qiuyang Zhang;Sen Liu;Dongxia Ge;Z. You
中科院分区：
文献类型：
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作者：
Lin Ma;Jingwu Li;Q. Nie;Qiuyang Zhang;Sen Liu;Dongxia Ge;Z. You
类器官模仿小器官的结构和功能。在过去的十年中，类器官培养技术已被所有研究团体迅速接受，用于研究干细胞，器官发育和功能以及患者特异性疾病。已经报道了用于人和小鼠前列腺上皮和癌组织的类器官培养的方案。然而，常用的人前列腺癌细胞系的类器官培养尚未建立。我们遵循公开的方案，在Matrigel TM和类器官培养基中进行LNCaP和C4-2B细胞的类器官培养14天。我们发现LNCaP和C4-2B细胞系都形成了呈现腺体结构的类器官。类器官内的细胞是雄激素受体阳性的腺癌细胞，但不是p63阳性的基底细胞。类器官中的细胞对白细胞介素-17A处理的反应与单层培养中的细胞不同。本研究表明，LNCaP和C4-2B细胞能够在规定的类器官培养条件下形成类器官。
Organoids mimic the architecture and functions of a small organ. Organoid culture technique has been rapidly accepted by all research communities during the past decade to study stem cells, organ development and function, and patient-specific diseases. A protocol for organoid culture of human and mouse prostate epithelial and cancer tissues has been reported. However, organoid culture of the commonly used human prostate cancer cell lines has yet to be established. We followed the published protocol and performed organoid culture of LNCaP and C4-2B cells in MatrigelTM and organoid culture medium for 14 days. We found that both LNCaP and C4-2B cell lines formed organoids that presented glandular structures. The cells within the organoids were androgen receptor-positive adenocarcinoma cells, but not p63-positive basal cells. The cells in the organoids responded to interleukin-17A treatment differently from the cells in the monolayer culture. The present study suggests that LNCaP and C4-2B cells are able to form organoids under the defined organoid culture conditions.