Tailoring the spectral response of liquid waveguide diagnostic platforms.
Tailoring the spectral response of liquid waveguide diagnostic platforms.
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DOI：
10.1002/jbio.201200049
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发表时间：
2012-08
影响因子：
2.8
通讯作者：
Hawkins AR
中科院分区：
文献类型：
--
作者：
Zhao Y;Phillips B;Ozcelik D;Parks J;Measor P;Gulbransen D;Schmidt H;Hawkins AR
形成芯片上生物光子学诊断平台的基础的液体填充波导主要应用于荧光和拉曼光谱学实验，其需要在弱分析物信号和各种背景信号之间进行灵敏的区分。背景信号的主要来源可以包括来自激发源的光（强、窄频带）和在波导包覆层中产生的光致发光（弱、宽频带）。在这里，我们审查固体和液体的核心过滤结构，这是基于抗共振反射，可以与波导集成衰减不需要的光学波段。考虑优化生物传感器的重要标准包括使广谱光致发光最小化并优化层厚度以在固体和液体引导层两者中产生期望的光谱响应的包覆层材料，以及能够产生具有可变光谱响应的区域的微制造工艺。新的研究结果描述了如何通过优化的热生长条件和如何可以调整液芯波导长度的液芯过滤器的歧视，可以最大限度地减少杂散荧光。
Liquid filled waveguides that form the basis for on-chip bio-photonics diagnostic platforms have primarily found application in fluorescence and Raman spectroscopy experiments that require sensitive discrimination between weak analyte signals and a variety of background signals. Primary sources of background signal can include light from excitation sources (strong, narrow frequency band) and photoluminescence generated in waveguide cladding layers (weak, wide frequency band). Here we review both solid and liquid core filtering structures which are based on anti-resonant reflection that can be integrated with waveguides for attenuating undesirable optical bands. Important criteria to consider for an optimized biosensor include cladding layer materials that minimize broad-spectrum photoluminescence and optimize layer thicknesses for creating a desired spectral response in both solid and liquid guiding layers, and a microfabrication process capable of producing regions with variable spectral response. New results describing how spurious fluorescence can be minimized by optimized thermal growth conditions and how liquid-core filter discrimination can be tuned with liquid core waveguide length are presented.