Peroxisome proliferator-activated receptor gamma is essential for stress adaptation by maintaining lipid homeostasis in female fish.
Peroxisome proliferator-activated receptor gamma is essential for stress adaptation by maintaining lipid homeostasis in female fish.
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DOI：
10.1016/j.bbalip.2022.159162
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发表时间：
2022-04
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影响因子：
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通讯作者：
Xue Wang;Qiang Ma;Ling-yun Chen;Hong-Xia Wu;Liqiao Chen;Fang Qiao;Yuan Luo;Meiling Zhang;Z. Du
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文献类型：
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作者：
Xue Wang;Qiang Ma;Ling-yun Chen;Hong-Xia Wu;Liqiao Chen;Fang Qiao;Yuan Luo;Meiling Zhang;Z. Du
Reduction of lipid synthesis often causes free fatty acid (FFA) overload, resulting consequential oxidative stress and health damage. Environmental stresses also induce cellular oxidative stress in organisms. The functional peroxisome proliferator-activated receptor gamma (pparg) gene is essential for lipid synthesis and homeostatic lipid maintenance. However, the relationship between thepparg-mediated lipid synthesis and environmental stress adaptation awaits full elucidation. Here, we generated apparg-knockout zebrafish model. The conversion of free fatty acids into triglycerides in the femaleppargmutants was hampered by reduced esterification efficiency, thus induced lipotoxicity, as evidenced by high oxidative stress and damaged health in these mutants, which led to reduced resistance to cold, heat and ammonia nitrogen stresses. Activatingppargin the wild-type female fish via dietary supplementation with rosiglitazone (appargagonist), or reducing oxidative stress in the femaleppargmutants via dietary supplementation withN-acetylcysteine (an antioxidant), or promoting mitochondrial fatty acid β-oxidation in the femaleppargmutants via dietary supplementation withl-carnitine, resulted in significantly reduced cellular injury, and improved environmental stress resistance. Collectively, our findings reveal that the regulative function ofppargin FFA esterification is important in stress resistance in female fish, and highlight the tight correlation existing between lipotoxicity and environmental adaptation.
