Acquisition of cytotoxic T lymphocyte-specific carbohydrate differentiation antigens.
Acquisition of cytotoxic T lymphocyte-specific carbohydrate differentiation antigens.
复制标题
获得细胞毒性T淋巴细胞特异性碳水化合物分化抗原的获取。
DOI：
10.1084/jem.162.4.1275
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发表时间：
1985-10-01
影响因子：
15.3
通讯作者：
Bevan, M J
中科院分区：
文献类型：
--
作者：
Lefrancois, L;Puddington, L;Machamer, C E;Bevan, M J
细胞毒性T细胞（CTL）特异性激活抗原，称为CT决定簇，已被检测到的单克隆抗体（mAb），抑制CTL功能。在细胞表面，CT抗原与T200糖蛋白和Mr为140，000和85，000的另外两种蛋白质结合，并存在于分泌蛋白gp 155上。高碘酸盐处理，然后结合分析和免疫沉淀实验，使用衣霉素处理的细胞表明，碳水化合物是必要的CT抗原表达。此外，gp 155在衣霉素存在下分泌，同时保留CT抗原，并且CT决定簇在T200生物合成后期加入，表明CT聚糖是O-连接的。最后，白细胞介素2显着影响的表达的CT单克隆抗体反应性寡糖存在于CTL细胞表面。
Cytotoxic T cell (CTL)-specific activation antigens, termed CT determinants, have been detected by monoclonal antibodies (mAb) that inhibit CTL function. At the cell surface, the CT antigens are associated with the T200 glycoproteins and two other proteins of Mr 140,000 and 85,000 and are present on a secreted protein, gp155. Periodate treatment followed by binding analysis and immunoprecipitation experiments using tunicamycin-treated cells indicated that carbohydrate is necessary for CT antigen expression. Furthermore, gp155 is secreted in the presence of tunicamycin while retaining the CT antigens, and the CT determinants are added late in T200 biosynthesis, suggesting that the CT glycans are O-linked. Finally, interleukin 2 was shown to dramatically influence the expression of the CT mAb-reactive oligosaccharides present at the CTL cell surface.