Human splenic galaptin: physicochemical characterization.
Human splenic galaptin: physicochemical characterization.
复制标题
人脾半乳糖苷：理化特性。
DOI：
10.1021/bi00474a014
复制
发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Allen,HJ
中科院分区：
文献类型：
--
作者：
Sharma,A;Chemelli,R;Allen,HJ
加拉哌丁的材料与方法。人脾在脾切除术中获得，并在20℃下保存直至使用。冷冻脾脏用钢锯切成小块，在10体积的冰水中匀浆，匀浆液在Buchner漏斗上过滤，残余物在20℃下保存直至利用。用asialofetuin-Sepharose亲和层析法从丙酮粉中分离出Galaptin
Materials and MethodsIsolation and Purity of Galaptin. Human spleen was ob-tained at splenectomy and stored at-20 C until utilized. Frozen spleen was cut into small fragments with the aid of a hacksaw and homogenized in 10 volumes of ice-cold ace-tone-0.01 Mme. 1 The homogenate was filtered on a Buchner funnel and the residue stored at-20 C until utilized. Galaptin was isolated from the acetone powder by affinity chromatography on asialofetuin-Sepharose essentially as