The BASIL cure: Using structure to predict function in protein biochemistry
The BASIL cure: Using structure to predict function in protein biochemistry
复制标题
BASIL 疗法：利用结构预测蛋白质生物化学中的功能
DOI：
10.1016/j.bpj.2022.11.1681
复制
发表时间：
2023
影响因子：
3.4
通讯作者：
Craig, Paul A.
中科院分区：
文献类型：
--
作者：
Koeppe, Julia R.;Roberts, Rebecca;Hall, Bonnie L.;Craig, Paul A.
星期二，2023年2月21日297 a功能。遵循BASIL课程的学生使用序列和结构比对工具的组合来研究这些结构，目的是识别可能的酶。然后，他们使用分子对接来预测什么样的模型底物适合接近拟议的活性位点。学生可以使用标准的湿实验室生物化学技术在实验室中生产目标酶进行表达和纯化，然后使用从对接研究中选择的模型底物进行动力学分析。我们已经成功地将这门课程用于专业和非专业的生物化学实验课程。课程是模块化的，可以作为一个整体使用，也可以将各个部分并入现有的课程-无论是讲座还是实验。课程材料可在basilbiochem免费获得。我们目前正在研究如何在各种学术环境中使用这门课程，我们欢迎新的合作者。我们可以通过虚拟会议提供同步支持，通过Slack提供异步支持。该项目得到了NSF IUSE 2141908的部分支持。
Tuesday, February 21, 2023 297a function. Students following the BASIL curriculum use a combination of sequence and structure alignment tools to study these structures with the goal of identifying possible enzymes. They then use molecular docking to predict what model substrates fit near a proposed active site. Students can produce the target enzymes in the lab using standard wet-lab biochemistry techniques for expression and purification, and they then perform kinetic assays with model substrates selected from their docking studies. We have successfully used this curriculum in biochemistry lab courses for majors and non-majors. The curriculum is modular and can be used as a whole or individual parts may be incorporated into an existing course-either lecture or lab. Curriculum materials are available free-of-charge at basilbiochem. org. We are currently investigating how this curriculum can be used in a variety of academic settings, and we welcome new collaborators. We can offer synchronous support via virtual meetings and asynchronous support via Slack. This project is supported in part by NSF IUSE 2141908.