Community structure and dynamics of the free and attached microorganisms during moderately thermophilic bioleaching of chalcopyrite concentrate.
Community structure and dynamics of the free and attached microorganisms during moderately thermophilic bioleaching of chalcopyrite concentrate.
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DOI：
10.1016/j.biortech.2010.04.003
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发表时间：
2010-09
影响因子：
11.4
通讯作者：
W. Zeng;G. Qiu;Hong-bo Zhou;J. Peng;Miao Chen;S. Tan;W. Chao;Xue-duan Liu;Yansheng Zhang
中科院分区：
文献类型：
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作者：
W. Zeng;G. Qiu;Hong-bo Zhou;J. Peng;Miao Chen;S. Tan;W. Chao;Xue-duan Liu;Yansheng Zhang
中等嗜热菌混合培养对黄铜矿的浸出效果良好。用扩增核糖体DNA限制性内切分析研究其群落结构。结果表明，浸出过程中主要检出嗜铁钩端螺旋体和嗜酸硫杆菌，唯一检出的古细菌为嗜热铁原体。因此，采用实时PCR法监测这些微生物在溶液和矿物表面的动态。结果表明，无论在溶液中还是在矿物表面，早期均以caldus为优势种，后期以L. ferriphilum为优势种。同一物种的数量在溶液和矿物表面表现出不同的变化趋势。附著嗜铁乳杆菌的数量增长快于游离乳杆菌的数量，并且嗜热乳杆菌的数量保持稳定，而游离乳杆菌的数量在最后迅速增加。
A mixed culture of moderately thermophiles showed good performance in bioleaching of chalcopyrite. Its community structure was investigated by amplified ribosomal DNA restriction analysis. The result shows that Leptospirillum ferriphilum and Acidithiobacillus caldus were the predominant bacteria and Ferroplasma thermophilum, the only archaea, could be always detected during bioleaching. Therefore, real-time PCR assay was performed to monitor these microorganisms’ dynamics in the solution and on the mineral surface. It is shown that both in the solution and on the mineral surface, A. caldus was the dominant species at the early stage and L. ferriphilum was the predominant species at the later stage. The amount of the same species exhibited different variation trends in the solution and on the mineral surface. The amount of attached L. ferriphilum increased faster than that of the free one, and the amount of attached F. thermophilum kept at a stable level while the free one increased rapidly at the end.