A novel rabbit model of mild, reproducible disc degeneration by an anulus needle puncture: Correlation between the degree of disc injury and radiological and histological appearances of disc degeneration
A novel rabbit model of mild, reproducible disc degeneration by an anulus needle puncture: Correlation between the degree of disc injury and radiological and histological appearances of disc degeneration
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DOI：
10.1097/01.brs.0000148152.04401.20
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发表时间：
2005-01-01
期刊：
影响因子：
3
通讯作者：
An, HS
中科院分区：
文献类型：
--
作者：
Masuda, K;Aota, Y;An, HS
研究设计.一项放射学和组织学评估诱导椎间盘退变的新方法的体内研究。通过用规定规格的针穿刺椎间盘环建立一种可重复的椎间盘退变兔模型，并将其与经典的穿刺模型进行比较。椎间盘退变的新治疗方法引起了极大的兴趣。虽然存在椎间盘退行性疾病的动物模型，但疾病进展的定量测量仍然困难。一个可重复的，渐进的椎间盘退变模型，它可以在合理的时间范围内诱导，对于开发新的治疗干预是必不可少的。采用经典的环形穿刺模型和新型的针刺模型。对于针刺模型，使用3种不同规格的针（16 G、18 G和21 G）对椎间盘造成5 mm深度的损伤。进行放射学和组织学分析;还在针刺模型中评估磁共振图像。在经典穿刺模型中，早在穿刺后2周就观察到明显的椎间隙狭窄;椎间隙没有进一步狭窄。在针穿刺模型中，所有测试的针尺寸均诱导椎间盘高度比经典穿刺模型更缓慢且更渐进地降低。磁共振成像支持椎间盘高度数据的结果。使用16 G至21 G针的针穿刺方法导致椎间盘高度和磁共振成像等级的可重复降低。手术的简便性和方法学的转移将使研究椎间盘退变的研究人员受益。
Study Design. An in vivo study to radiographically and histologically assess a new method of induction of disc degeneration.Objective. To establish a reproducible rabbit model of disc degeneration by puncturing the anulus with needles of defined gauges and to compare it to the classic stab model.Summary of Background Data. New treatment approaches to disc degeneration are of great interest. Although animal models for disc degenerative disease exist, the quantitative measurement of disease progression remains difficult. A reproducible, progressive disc degeneration model, which can be induced in a reasonable time frame, is essential for development of new therapeutic interventions.Methods. The classic anular stab model and the new needle puncture model were used in the rabbit. For the needle puncture model, 3 different gauges of needle (16G, 18G, and 21G) were used to induce an injury to the disc to a depth of 5 mm. Radiographic and histologic analyses were performed; magnetic resonance images were also assessed in the needle puncture model.Results. Significant disc space narrowing was observed as early as 2 weeks after stabbing in the classic stab model; there was no further narrowing of the disc space. In the needle puncture model, all needle sizes tested induced a slower and more progressive decrease in disc height than in the classic stab model. The magnetic resonance imaging supported the results of disc height data.Conclusions. The needle puncture approach, using 16G to 21G needles, resulted in a reproducible decrease of disc height and magnetic resonance imaging grade. The ease of the procedure and transfer of the methodology will benefit researchers studying disc degeneration.