Evaluation of a Reformulated CHROMagar Candida
Evaluation of a Reformulated CHROMagar Candida
复制标题
重新配制的科玛嘉念珠菌的评估
DOI：
10.1128/jcm.39.5.2015-2016.2001
复制
发表时间：
2001
影响因子：
9.4
通讯作者：
T. Meiller
中科院分区：
文献类型：
--
作者：
M. Jabra;Troy M. Brenner;Mark J. Romagnoli;A. Baqui;W. Merz;W. Falkler;T. Meiller
CHROMagar Candida是一种用于分离和推定鉴定临床重要酵母菌的鉴别培养基。最近，Becton Dickinson重新制定了该培养基。本研究旨在评估CHROMagar新配方针对原始CHROMagar念珠菌的回收率、生长和菌落颜色以及储备培养物和临床标本直接平板接种的性能。本研究共纳入了代表9种酵母菌种（包括都柏林念珠菌）的90株储备酵母分离株以及522份临床标本。对于大多数物种来说，两种培养基之间的生长率或菌落大小没有显着差异。然而，评价的所有10株白色念珠菌分离株在新的CHROMagar制剂上始终显示较浅的绿色。相比之下，所有26 C。Dubliniensis分离株在两种培养基上均呈现相同的典型深绿色。在522份临床标本中，共有173份酵母菌呈阳性，回收了8种酵母菌。在两种培养基上，各菌种的回收率相当，菌落大小或颜色无一致的菌种相关差异。虽然两种培养基的性能相当，但C.新的CHROMagar上的白色念珠菌分离株使其更容易区分C.白色念珠菌和C.都柏林分离物。总之，新配制的Becton Dickinson CHROMagar念珠菌培养基与原始CHROMagar念珠菌培养基同样适合作为鉴别培养基，用于酵母菌菌种的推定鉴定和临床标本中多种酵母菌菌种的检测。
ABSTRACT CHROMagar Candida is a differential culture medium for the isolation and presumptive identification of clinically important yeasts. Recently the medium was reformulated by Becton Dickinson. This study was designed to evaluate the performance of the new formula of CHROMagar against the original CHROMagar Candida for recovery, growth, and colony color with stock cultures and with direct plating of clinical specimens. A total of 90 stock yeast isolates representing nine yeast species, including Candida dubliniensis, as well as 522 clinical specimens were included in this study. No major differences were noted in growth rate or colony size between the two media for most of the species. However, all 10 Candida albicans isolates evaluated consistently gave a lighter shade of green on the new CHROMagar formulation. In contrast, all 26 C. dubliniensis isolates gave the same typical dark green color on both media. A total of 173 of the 522 clinical specimens were positive for yeast, with eight yeast species recovered. The recovery rates for each species were equivalent on both media, with no consistent species-associated differences in colony size or color. Although both media were comparable in performance, the lighter green colonies ofC. albicans isolates on the new CHROMagar made it easier to differentiate between C. albicans and C. dubliniensis isolates. In conclusion, the newly formulated Becton Dickinson CHROMagar Candida medium is as equally suited as a differential medium for the presumptive identification of yeast species and for the detection of multiple yeast species in clinical specimens as the original CHROMagar Candida medium.