Alkaliphilic and halophilic hydrocarbon-utilizing bacteria from Kuwaiti coasts of the Arabian Gulf
Alkaliphilic and halophilic hydrocarbon-utilizing bacteria from Kuwaiti coasts of the Arabian Gulf
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DOI：
10.1007/s00253-007-1127-1
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发表时间：
2007-11-01
影响因子：
5
通讯作者：
Radwan, S. S.
中科院分区：
文献类型：
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作者：
Al-Awadhi, H.;Sulaiman, Rasha H. D.;Radwan, S. S.
阿拉伯湾沿岸潮间带的绿色有生命的物质比无生命的物质容纳了更多的嗜碱和嗜盐细菌。根据16 S核糖核酸序列鉴定，嗜碱石油利用细菌属于以下按频率递减排列的属：海杆菌属、微球菌属、迪茨菌属、芽孢杆菌属、海洋芽孢杆菌属和柠檬球菌属。嗜盐石油利用菌属为：海海扁藻属、Georgenia属、微杆菌属、Stappia属、芽孢杆菌属、等翅菌属和纤维单胞菌属。大多数菌株可以生长在广泛的纯正构烷烃和芳香族化合物，作为唯一的碳源和能源。定量气液色谱分析表明，单个分离株削弱了培养物中的原油和代表性纯碳氢化合物。大多数嗜碱菌属的最适pH为10，嗜盐菌的最适盐度范围为2.5和5%NaCl（w/v）。得出的结论是，就其微生物组成而言，科威特海岸的油性碱性和盐碱潮间带具有自我清洁的潜力。
Green animate materials from the intertidal zone of the Arabian Gulf coast accommodated more alkaliphilic and halophilic bacteria than inanimate materials. The alkaliphilic oil-utilizing bacteria, as identified by their 16S ribonucleic acid sequences, belonged to the following genera arranged in decreasing frequences: Marinobacter, Micrococcus, Dietzia, Bacillus, Oceanobacillus, and Citricoccus. The halophilic oil-utilizing bacteria belonged to the genera: Marinobacter, Georgenia, Microbacterium, Stappia, Bacillus, Isoptericola, and Cellulomonas. Most isolates could grow on a wide range of pure n-alkanes and aromatic compounds, as sole sources of carbon and energy. Quantitative gas liquid chromatographic analysis showed that individual isolates attenuated crude oil and representative pure hydrocarbons in culture. The optimum pH for most of the alkaliphilic genera was pH 10, and the optimum salinity for the halophiles ranged between 2.5 and 5% NaCl (w/v). It was concluded that as far as their microbial makeup is concerned, oily alkaline and saline intertidal areas of the Kuwaiti coasts have a self-cleaning potential.