A score method for comparison of partial genomic regions in their representatives of full-length genome of hepatitis E virus for genotyping
A score method for comparison of partial genomic regions in their representatives of full-length genome of hepatitis E virus for genotyping
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DOI：
10.1159/000106805
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发表时间：
2007-01-01
期刊：
影响因子：
4.6
通讯作者：
Meng, Jihong
中科院分区：
文献类型：
--
作者：
Xun, Pengcheng;Chen, Feng;Meng, Jihong
建立了戊型肝炎病毒（HEV）全长基因组部分基因组区域代表性比较的统计评分法。假设检验的统计量的临界值是通过蒙特卡罗模拟产生的。发现片段III可能是HEV全基因组基因分型的统计代表性区域。基于全长序列和片段III的系统发育分析结果表明，HEV基因型相同，在基因型、亚型和分离株3个水平上的平均核苷酸差异范围相似，进一步验证了统计学结果。结果表明，评分法可有效地用于HEV株部分基因组区域与全长基因组区域的比较，以进行基因分型。版权所有（c）2007 S. Karger AG，巴塞尔。
In this article, a statistical score method for comparison of partial genomic regions in their representatives of full-length genome of hepatitis E virus (HEV) for genotyping was developed. The critical values of the statistics for hypothesis testing were generated through Monte Carlo simulations. It was found that fragment III might be a statistically representative region for the whole genome of HEV for genotyping. Based on both the full-length sequence and fragment III, the same four genotypes were identified by the subsequent phylogenetic analysis, and similar ranges of mean nucleotide difference were also observed to differentiate HEV sequences at the three levels genotype, subtype and isolate, which further verified the statistical results. It may be concluded from this study that the score method is effective for comparing partial genomic regions with full-length genome of HEV strains for genotyping. Copyright (c) 2007 S. Karger AG, Basel.