Selective cholinergic modulation of cortical GABAergic cell subtypes
Selective cholinergic modulation of cortical GABAergic cell subtypes
复制标题
DOI：
10.1152/jn.1997.78.3.1743
复制
发表时间：
1997-09-01
影响因子：
2.5
通讯作者：
Kawaguchi, Y
中科院分区：
文献类型：
--
作者：
Kawaguchi, Y
来自基底前脑的乙酰胆碱和来自皮质内抑制性中间神经元的γ-氨基丁酸（GABA）对皮质活动产生强烈影响，并可能相互作用。胆碱能或毒蕈碱激动剂确实通过兴奋抑制性中间神经元诱导锥体细胞中的GABA能突触后电流，这些中间神经元最近根据生理学、化学和形态学标准被分为几个不同的亚型。采用离体全细胞记录和细胞内染色技术，研究了胆碱能对幼年大鼠额叶皮层GABA能细胞亚型的影响。GABA能细胞亚型通过对去极化电流脉冲的放电反应进行生理学鉴定，化学鉴定为含有小清蛋白、生长抑素、血管活性肠多肽（VIP）或胆囊收缩素（CCK）。卡巴胆碱（10 μ M）或（+）-毒蕈碱（3 μ M）影响具有规则或爆发性尖峰特征的含肽GABA能细胞的活性，但不影响具有快速尖峰特征的含钙结合蛋白小清蛋白的GABA能细胞或具有迟尖峰特征的GABA能细胞的活性。生长抑素或VIP免疫反应细胞去极化与尖峰放电。CCK免疫反应细胞受到胆碱能激动剂的不均匀影响。较大的CCK细胞超极化，然后缓慢去极化，而较小的CCK细胞仅去极化。这些结果表明，皮层GABA能细胞亚型的兴奋性是由乙酰胆碱差异调节。胆碱能反应的差异表明每个GABA能细胞亚型的不同功能作用。
Acetylcholine from the basal forebrain and gamma-aminobutyric acid (GABA) from intracortical inhibitory interneurons exert strong influence on the cortical activity and may interact with each other. Cholinergic or muscarinic agonists indeed induced GABAergic postsynaptic currents in pyramidal cells by exciting inhibitory interneurons that have recently been classified into several distinct subtypes on the basis of the physiological, chemical, and morphological criteria. Cholinergic effects on GABAergic cell subtypes were investigated of rat frontal cortex by in vitro whole cell recording with intracellular staining in frontal cortex of young rats. GABAergic cell subtypes were identified physiologically by firing responses to depolarizing current pulses and immunohistochemically as containing parvalbumin, somatostatin, vasoactive intestinal polypeptide (VIP), or cholecystokinin (CCK). Carbachol (10 mu M) or (+)-muscarine (3 mu M) affected the activities of peptide-containing GABAergic cells with regular-or burst-spiking characteristics, but not of GABAergic cells with fast-spiking characteristics containing the calcium-binding protein parvalbumin or GABAergic cells with late-spiking characteristics. Somatostatin- or VIP-immunoreactive cells were depolarized with spike firing. CCK-immunoreactive cells were affected heterogeneously by cholinergic agonists. Larger CCK cells were hyperpolarized, followed by a slow depolarization, whereas smaller CCK cells were only depolarized. These results suggest that the excitability of cortical GABAergic cell subtypes is differentially regulated by acetylcholine. Differences in cholinergic responses suggest a distinct functional role of each GABAergic cell subtype.