The development and application of high throughput cultivation technology in bioprocess development
The development and application of high throughput cultivation technology in bioprocess development
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DOI：
10.1016/j.jbiotec.2014.03.028
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发表时间：
2014-12-20
影响因子：
4.1
通讯作者：
Bai, Zhonghu
中科院分区：
文献类型：
--
作者：
Long, Quan;Liu, Xiuxia;Bai, Zhonghu
本文综述了高通量（HTP）培养技术的最新进展及其在质量设计（QbD）驱动的生物工艺开发中的应用。几个实用的HTP策略旨在缩短工艺开发（PD）时间表，从DNA到涉及商用HTP技术平台的大规模工艺，包括微滴板（MTP）培养，微规模生物反应器，并行发酵系统等，都进行了详细的审查。本文主要讨论在这种情况下这些平台的相对优势和劣势或局限性。最近报道的微型生物反应器原型，如毫升（mL）规模的搅拌槽生物反应器和微流体集成微型生物反应器，以及它们在qbd驱动的HTP工艺开发中的实际应用潜力也进行了批判性评价。这种技术的总体目标是快速获得过程洞察力，并且由于在HTP系统中部署的分析技术对实现这一目标至关重要，因此讨论了这一快速发展的领域。最后，对未来的总体趋势进行了严格的审查。
This review focuses on recent progress in the technology of high throughput (HTP) cultivation and its increasing application in quality by design (QbD) -driven bioprocess development. Several practical HTP strategies aimed at shortening process development (PD) timelines from DNA to large scale processes involving commercially available HTP technology platforms, including microtiter plate (MTP) culture, micro-scale bioreactors, and in parallel fermentation systems, etc., are critically reviewed in detail. This discussion focuses upon the relative strengths and weaknesses or limitations of each of these platforms in this context. Emerging prototypes of micro-bioreactors reported recently, such as milliliter (mL) scale stirred tank bioreactors, and microfludics integrated micro-scale bioreactors, and their potential for practical application in QbD-driven HTP process development are also critically appraised. The overall aim of such technology is to rapidly gain process insights, and since the analytical technology deployed in HTP systems is critically important to the achievement of this aim, this rapidly developing area is discussed. Finally, general future trends are critically reviewed.