THE PURIFICATION OF LACTOFERRIN FROM HUMAN WHEY BY BATCH EXTRACTION
THE PURIFICATION OF LACTOFERRIN FROM HUMAN WHEY BY BATCH EXTRACTION
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DOI：
10.1016/0003-2697(87)90040-6
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发表时间：
1987-04-01
影响因子：
2.9
通讯作者：
BATES, GW
中科院分区：
文献类型：
--
作者：
FOLEY, AA;BATES, GW
从人乳清中分离乳铁蛋白是一种快速的两步法。乳铁蛋白通过分批萃取直接吸附到磷酸纤维素上，并通过阶梯盐和pH梯度洗脱。主要的杂质是低分子量的部分，通过凝胶过滤可以迅速去除。回收的乳铁蛋白纯度约为96%。乳铁蛋白的平均产率为80%。这种乳铁蛋白纯化方法大大减少了所需的劳动和时间，并且该过程很容易缩放到任何体积的起始材料。
The isolation of lactoferrin from human whey has been accomplished using a rapid two-step procedure. The lactoferrin is directly adsorbed to cellulose phosphate by batch extraction and eluted by a stepped salt and pH gradient. The major impurity, a low-molecular-weight fraction, is quickly removed by gel filtration. The recovered lactoferrin has a purity of about 96%. The yield of lactoferrin averaged 80%. This method of lactoferrin purification greatly reduces the labor and time required, and the procedure is easily scaled to any volume of starting material.