Behavioral arousal blocks light-induced phase advances in locomotor rhythmicity but not light-induced Per1 and fos expression in the hamster suprachiasmatic nucleus
Behavioral arousal blocks light-induced phase advances in locomotor rhythmicity but not light-induced Per1 and fos expression in the hamster suprachiasmatic nucleus
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DOI：
10.1016/s0306-4522(02)00908-9
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发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
Mrosovsky, N
中科院分区：
文献类型：
--
作者：
Edelstein, K;De La Iglesia, HO;Mrosovsky, N
光刺激和非光刺激都引起昼夜节律。虽然无光时钟重置的适应意义是未知的，一种可能性是，无光线索调节昼夜节律对光的反应。光刺激和非光刺激之间相互作用的研究结果支持了这一观点。在白天，光阻断了非光刺激对运动节律的相位和对视交叉上核（SCN）神经元中时钟基因表达的影响。在晚上，新奇诱导的活动之前和期间暴露于光衰减相移响应，光，但这种操纵时钟基因表达的影响是未知的。本实验在分子水平上探索行为状态与光反应之间的相互作用。我们发现，在主观的深夜光脉冲后立即将仓鼠限制在新的车轮上，会减弱光诱导的车轮运行节奏的相位提前和对昼夜节律的瞬态影响。与新奇诱导的活动对光的行为反应的显著影响相反，Fos蛋白和Per1 mRNA在所有光脉冲动物的SCN中强烈表达，无论行为治疗如何。我们的研究结果是不一致的想法，光和nonphotic刺激阻止对方的影响相移诱导或衰减转录的Per1。光对生物钟基因的调控和生物钟基因的自发节律性表达可能是通过不同的机制介导的。(C)2003年IBRO。由爱思唯尔科技有限公司出版。保留所有权利。
Both photic and nonphotic stimuli entrain circadian rhythms. Although the adaptive significance of nonphotic clock resetting is unknown, one possibility is that nonphotic cues modulate circadian responses to light. Results of studies on the interaction between photic and nonphotic stimuli support this idea. During the day, light blocks the effects of nonphotic stimuli on the phase of locomotor rhythms and on expression of clock genes in suprachiasmatic nucleus (SCN) neurons. At night, novelty-induced activity prior to and during exposure to light attenuates the phase-shifting response to that light, but the effects of this manipulation on clock gene expression are unknown. The present experiments explore the interaction between behavioral state and response to light at the molecular level. We show that confining hamsters to novel wheels immediately after a light pulse during the late subjective night attenuates light-induced phase advances of wheel-running rhythms and the transient effects on circadian period. In contrast to the striking effect of novelty-induced activity on behavioral responses to light, Fos protein and Per1 mRNA were robustly expressed in the SCN of all light-pulsed animals, regardless of behavioral treatment. Our results are inconsistent with the idea that light and nonphotic stimuli block each other's effects on phase shifts by inducing or attenuating transcription of Per1. Photic regulation of clock genes and spontaneous rhythmic expression of clock genes are probably mediated by different mechanisms. (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.