Protective effects of myricitrin against osteoporosis via reducing reactive oxygen species and bone-resorbing cytokines
Protective effects of myricitrin against osteoporosis via reducing reactive oxygen species and bone-resorbing cytokines
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DOI：
10.1016/j.taap.2014.08.004
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发表时间：
2014-11-01
影响因子：
3.8
通讯作者：
Liu, Jian
中科院分区：
文献类型：
--
作者：
Huang, Qiang;Gao, Bo;Liu, Jian
氧化应激是骨质疏松症发生发展的重要致病因素。杨梅苷是一种有效的抗氧化剂，从杨梅中分离得到。我们假设杨梅苷具有抗骨质疏松的保护作用，部分减少活性氧（ROS）和骨吸收细胞因子在成骨细胞MC 3 T3-E1细胞和人骨髓基质细胞（hBMSCs）。我们研究了杨梅苷对氧化应激下成骨分化的影响。采用过氧化氢（H2 O2）建立氧化性细胞损伤模型。我们的研究结果表明，杨梅苷显着提高这些细胞中的一些成骨标志物。杨梅苷降低hBMSCs中脂质生成和过氧化物酶体增殖物激活受体γ 2（PPAR γ 2）表达。此外，杨梅苷降低核因子κ B受体激活因子配体（RANKL）和IL-6的表达，并部分抑制ROS的产生。在体内，我们建立了小鼠卵巢切除（OVX）骨质疏松症模型。我们的研究结果表明，杨梅苷补充降低血清丙二醛（MDA）的活性和增加还原型谷胱甘肽（GSH）的活性。重要的是，它改善了第4腰椎（L4）和股骨远端的骨小梁的微结构。综上所述，这些结果表明杨梅苷对骨质疏松症的保护作用与ROS和骨吸收细胞因子的减少有关，表明杨梅苷可用于骨代谢疾病，特别是骨质疏松症。(C)版权所有© 2014 Elsevier Inc.
Oxidative stress is a crucial pathogenic factor in the development of osteoporosis. Myricitrin, isolated from Myrica cerifera, is a potent antioxidant. We hypothesized that myricitrin possessed protective effects against osteoporosis by partially reducing reactive oxygen species (ROS) and bone-resorbing cytokines in osteoblastic MC3T3-E1 cells and human bone marrow stromal cells (hBMSCs). We investigated myricitrin on osteogenic differentiation under oxidative stress. Hydrogen peroxide (H2O2) was used to establish an oxidative cell injury model. Our results revealed that myricitrin significantly improved some osteogenic markers in these cells. Myricitrin decreased lipid production and reduced peroxisome proliferator-activated receptor gamma-2 (PPAR gamma 2) expression in hBMSCs. Moreover, myricitrin reduced the expression of receptor activator of nuclear factor kappa-B ligand (RANKL) and IL-6 and partially suppressed ROS production. In vivo, we established a murine ovariectomized (OVX) osteoporosis model. Our results demonstrated that myricitrin supplementation reduced serum malondialdehyde (MDA) activity and increased reduced glutathione (GSH) activity. Importantly, it ameliorated the micro-architecture of trabecular bones in the 4th lumbar vertebrae (L4) and distal femur. Taken together, these results indicated that the protective effects of myricitrin against osteoporosis are linked to a reduction in ROS and bone-resorbing cytokines, suggesting that myricitrin may be useful in bone metabolism diseases, particularly osteoporosis. (C) 2014 Elsevier Inc All rights reserved.