Expression of the angiogenic growth factors VEGF, FGF-2, EGF and their receptors in normal human endometrium during the menstrual cycle
Expression of the angiogenic growth factors VEGF, FGF-2, EGF and their receptors in normal human endometrium during the menstrual cycle
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DOI：
10.1093/molehr/7.1.65
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发表时间：
2001-01-01
影响因子：
4
通讯作者：
Olovsson, M
中科院分区：
文献类型：
--
作者：
Möller, B;Rasmussen, C;Olovsson, M
血管生成是循环子宫内膜的重要但知之甚少的过程，子宫内膜血管生成被认为受卵巢类固醇影响的血管生长因子的调节。血管内皮生长因子（VEGF）及其受体VEGFR-1和VEGFR-2，成纤维细胞生长因子2（FGF-2）及其受体FGFR-1和FGFR-2，以及表皮生长因子（EGP）及其受体。据信EGFR在人类子宫内膜的血管生成中很重要，它们的表达是通过子宫内膜中的免疫组织化学检查的从16位健康妇女获得的活检。蛋白质印迹分析表明，所使用的主要抗体是特异性的。我们发现VEGF，FGF-2，EGF及其受体均表达，尤其是在血管中和/或周围，因此支持了这些肽有助于调节人体子宫内膜中血管生成和血管功能的假说VEGFR-1，VEGFR-2，FGFR-2和EGFR共表达，并在分泌阶段开始时表现出最强的表达子宫内膜水肿和复杂的上皮下毛细血管丛的形成。受体表达与基质血管密度之间没有看到相关性。
Angiogenesis is an important but poorly understood process of the cycling endometrium, Endometrial angiogenesis is believed to be regulated by angiogenic growth factors under the influence of ovarian steroids. Vascular endothelial growth factor (VEGF) and its receptors VEGFR-1 and VEGFR-2, fibroblast growth factor 2 (FGF-2) and its receptors FGFR-1 and FGFR-2, as well as epidermal growth factor (EGP) and its receptor EGFR are believed to be important in the control of angiogenesis in the human endometrium, Their expression was examined by immunohistochemistry in endometrial biopsies obtained from 16 healthy women with proven fertility. Western blot analysis showed that the primary antibodies used were specific for their epitopes. We found that VEGF, FGF-2, EGF and their receptors were all expressed, especially in and/or around blood vessels, thus supporting the hypothesis that these peptides contribute to the regulation of angiogenesis and blood vessel function in the human endometrium, The receptors VEGFR-1, VEGFR-2, FGFR-2 and EGFR were co-expressed and exhibited their strongest expression during the beginning of the secretory phase, coinciding with the developing endometrial oedema and formation of a complex subepithelial capillary plexus. No correlation was seen between receptor expression and stromal blood vessel density.