Techniques for high-speed cardiac magnetic resonance imaging in rats and rabbits.
Techniques for high-speed cardiac magnetic resonance imaging in rats and rabbits.
复制标题
大鼠和兔子的高速心脏磁共振成像技术。
DOI：
10.1002/mrm.1910370118
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发表时间：
1997
影响因子：
3.3
通讯作者：
Judd,RM
中科院分区：
文献类型：
--
作者：
Rehwald,WG;Reeder,SB;McVeigh,ER;Judd,RM
近年来，通过选择性育种和/或基因改变建立专门的小动物模型，极大地促进了高血压、心力衰竭、衰老、梗死后重构和心血管疾病分子基础的研究进展。然而，使用现有方法很难对这些动物模型的心功能和血流灌注进行常规的无创性评估。原则上，核磁共振可以用于这一目的，但在实践中，这是困难的，因为与射频线圈、心脏门控和成像脉冲序列有关的问题。在这篇文章中，我们描述了这些问题的解决方案，使我们能够使用MRI常规地对大鼠和兔子的心脏进行成像。具体描述了四个RF线圈、心脏门控方案以及专门为在4.7T欧米茄化学位移成像(CSI)光谱仪上进行心脏成像而设计的成像脉冲序列。这些技术可在2分钟内获得8幅大鼠心脏不同时相的双斜短轴位图像，平面分辨率为200×400μm，层厚为2 mm。此外，心肌组织标记可以用与人类常规使用的标记厚度和间隔相当的标记来执行。提供的技术信息足够详细，使其他站点的研究人员能够复制结果。这些信息应该有助于使用MRI进行心脏功能和血流灌注的非侵入性检查，这可以与其他已建立的技术相结合，在专门的动物模型中研究心血管疾病。
Progress in research on hypertension, heart failure, aging, post‐infarct remodeling, and the molecular basis of cardiovascular diseases in general has been greatly facilitated in recent years by the development of specialized small‐mam‐mal models by selective breeding and/or genetic alteration. Routine noninvasive evaluation of cardiac function and perfusion in these animals models, however, is difficult using existing methods. In principle, MRI can be used for this purpose, but in practice this is difficult because of problems related to RF coils, cardiac gating, and imaging pulse sequences. In this article, solutions to these problems are described that have allowed us to use MRI to routinely image the hearts of rats and rabbits. Specifically described are four RF coils, cardiac gating schemes, and an imaging pulse sequence specially designed for cardiac imaging in these animals on a 4.7 T Omega chemical‐shift imaging (CSI) spectrometer. These techniques can be used to obtain, within 2 min, eight double‐oblique short‐axis images of the rat at different cardiac phases with 200 × 400μm in‐plane resolution and a slice thickness of 2 mm. Moreover, myocardial tissue tagging can be performed with tag thicknesses and separations comparable to those used routinely in humans. The technical information is presented in sufficient detail to allow researchers at other sites to reproduce the results. This information should facilitate the use of MRI for the noninvasive examination of cardiac function and perfusion, which can be combined with other established techniques for the study of cardiovascular disease in specialized animal models.