Expression analysis of rice VQ genes in response to biotic and abiotic stresses
Expression analysis of rice VQ genes in response to biotic and abiotic stresses
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DOI：
10.1016/j.gene.2013.08.023
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发表时间：
2013-10-01
期刊：
影响因子：
3.5
通讯作者：
Hwang, D. J.
中科院分区：
文献类型：
--
作者：
Kim, D. Y.;Kwon, S. I.;Hwang, D. J.
WRKY转录因子在植物中由一个大的基因超家族编码，具有广泛的作用。含有短VQ（FxxxVQxLTG）基序的蛋白质最近被证明与WRKY转录因子相互作用，这意味着AtVQ蛋白在拟南芥的植物防御反应中是重要的，无论是作为WRKY转录因子的正或负辅因子。通过全基因组分析，鉴定了39个含有VQ基序的水稻基因（OsVQs），并对OsVQ蛋白的基因组结构进行了分析。此外，与拟南芥和水稻的VQ结构域进行系统发育树分析。这些OsVQ基因在几种胁迫处理下的植物中的表达模式进行了评估，具体地说，在感染细菌病原体黄单胞菌致病型。用水稻原生质体系统检测了几个OsVQ蛋白的细胞定位。基于我们的研究结果，我们认为OsVQ蛋白在植物对生物和非生物胁迫的防御反应中起着重要的辅助调节作用。(c)2013爱思唯尔有限公司版权所有。
WRKY transcription factors are encoded by a large gene superfamily with a broad range of roles in plants. Proteins containing a short VQ (FxxxVQxLTG) motif have been recently shown to interact with WRKY transcription factors, implying that AtVQ proteins are important in the plant defense responses in Arabidopsis, either as positive or negative cofactors of WRKY transcription factors. Thirty-nine Oryza sativa genes containing the VQ motif (OsVQs) were identified and the genome structures of OsVQ proteins were characterized through genome-wide analysis in rice. Also, phylogenetic tree analysis was performed with the VQ domain of Arabidopsis and rice. The expression patterns of these OsVQ genes in plants under several stress treatments were assessed, specifically, following infection with the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo), treatment with abscisic acid (ABA), or exposure to drought The cellular localization of a few OsVQ proteins was examined using rice protoplast system. Based on our results, we suggest that OsVQ proteins function as important co-regulators during the plant defense response to biotic and abiotic stresses. (c) 2013 Elsevier B.V. All rights reserved.