A Spatiotemporal Organ-Wide Gene Expression and Cell Atlas of the Developing Human Heart
A Spatiotemporal Organ-Wide Gene Expression and Cell Atlas of the Developing Human Heart
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DOI：
10.1016/j.cell.2019.11.025
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发表时间：
2019-12-12
期刊：
影响因子：
64.5
通讯作者：
Lundeberg, Joakim
中科院分区：
文献类型：
--
作者：
Asp, Michaela;Giacomello, Stefania;Lundeberg, Joakim
人类心脏形态发生的过程还不完全清楚。它的完整表征需要深入探索具有单细胞空间分辨率的全器官基因表达编排。在这里，我们提出了一种分子方法，揭示了全面的转录景观的细胞类型的人口在三个发育阶段的胚胎心脏，并映射细胞类型特异性基因表达的特定解剖结构域。空间转录组学鉴定了对应于每个发育阶段不同解剖区域的独特基因谱。通过单细胞RNA测序鉴定的人胚胎心脏细胞类型证实并丰富了胚胎心脏基因表达的空间注释。然后使用原位测序来完善这些结果，并创建三个发育阶段的空间亚细胞图谱。最后，我们建立了一个公开的人类心脏发育的网络资源，以促进未来对人类心脏发生的研究。
The process of cardiac morphogenesis in humans is incompletely understood. Its full characterization requires a deep exploration of the organ-wide orchestration of gene expression with a single-cell spatial resolution. Here, we present a molecular approach that reveals the comprehensive transcriptional landscape of cell types populating the embryonic heart at three developmental stages and that maps cell-type-specific gene expression to specific anatomical domains. Spatial transcriptomics identified unique gene profiles that correspond to distinct anatomical regions in each developmental stage. Human embryonic cardiac cell types identified by single-cell RNA sequencing confirmed and enriched the spatial annotation of embryonic cardiac gene expression. In situ sequencing was then used to refine these results and create a spatial subcellular map for the three developmental phases. Finally, we generated a publicly available web resource of the human developing heart to facilitate future studies on human cardiogenesis.