Antimicrobial Susceptibilities and Resistance Genes of Canadian Isolates of Actinobacillus pleuropneumoniae
Antimicrobial Susceptibilities and Resistance Genes of Canadian Isolates of Actinobacillus pleuropneumoniae
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DOI：
10.1089/mdr.2011.0150
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发表时间：
2012-04-01
影响因子：
2.6
通讯作者：
Jacques, Mario
中科院分区：
文献类型：
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作者：
Archambault, Marie;Harel, Josee;Jacques, Mario
胸膜肺炎放线杆菌是猪胸膜肺炎的病原体，胸膜肺炎是一种严重的、高度接触性的呼吸道疾病，在世界范围内造成养猪业的经济损失。虽然A.胸膜肺炎最近已在不同国家报告，加拿大目前的情况尚不清楚。本研究的目的是测定43株A.胸膜肺炎分离于加拿大。此外，用寡核苷酸芯片检测抗菌药物耐药基因。还评价了生物膜形成对抗菌药物敏感性的影响。所有分离株均对头孢噻呋、氟苯尼考、恩诺沙星、红霉素、克林霉素、甲氧苄啶/磺胺甲恶唑和替米考星敏感。观察到对泰妙菌素、青霉素、氨苄西林以及达氟沙星的低水平耐药。我们观察到高水平的耐药金霉素（88.4%）和土霉素（90.7%）。对四环素类抗生素显示耐药性的菌株含有至少一种以下tot基因：tetB、tetO、tetH或tetC。5株分离株对青霉素类（bla（ROB-1））、链霉素[aph 3“”（strA）]、磺胺类（sulII）和四环素类（tetO）抗生素显示多重耐药性，而另外3株分离株对链霉素[aph 3“”（strA）]、磺胺类（sulII）和四环素类（tetB、tetO或tetB/tetH）抗生素显示多重耐药性。据我们所知，这是第一个描述的tetC基因在巴斯德菌科。最后，A.生物膜中的胸膜肺炎患者对抗菌药物的耐药性比他们的肺炎同行高100至30，000倍。
Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, a severe and highly contagious respiratory disease responsible for economic losses in the swine industry worldwide. Although antimicrobial resistance in A. pleuropneumoniae has been recently reported in different countries, the current situation in Canada is unknown. The aim of the current study was to determine the antimicrobial susceptibilities of 43 strains of A. pleuropneumoniae isolated in Canada. In addition, antimicrobial resistance genes were detected with an oligonucleotide microarray. The impact of biofilm formation on susceptibility to antimicrobials was also evaluated. All isolates were susceptible to ceftiofur, florfenicol, enrofloxacin, erythromycin, clindamycin, trimethoprim/sulfamethoxazole, and tilmicosin. A low level of resistance was observed toward tiamulin, penicillin, and ampicillin as well as danofloxacin. We observed a high level of resistance to chlortetracycline (88.4%) and oxytetracycline (90.7%). The strains showing resistance to tetracycline antimicrobials contained at least one of the following tot genes: tetB, tetO, tetH, or tetC. Five isolates showed multiresistance to penicillins (bla(ROB-1)), streptomycin [aph3 '' (strA)], sulfonamides (sulII), and tetracyclines (tetO) antimicrobials whereas three others showed multiresistance to streptomycin [aph3 '' (strA)], sulfonamides (sulII), and tetracyclines (tetB, tetO, or tetB/tetH) antimicrobials. To the best of our knowledge, this is the first description of tetC gene in Pasteurellaceae. Finally, cells of A. pleuropneumoniae in a biofilm were 100 to 30,000 times more resistant to antimicrobials than their planktonic counterparts.