Endogenous L-Carnosine Level in Diabetes Rat Cardiac Muscle.
Endogenous L-Carnosine Level in Diabetes Rat Cardiac Muscle.
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DOI：
10.1155/2016/6230825
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Song Y
中科院分区：
文献类型：
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作者：
Liu Y;Su D;Zhang L;Wei S;Liu K;Peng M;Li H;Song Y
描述了一种使用HPLC-UV定量心肌肌肽水平的新方法。用乙腈沉淀蛋白质，提取大鼠心肌肌肽和内标胸腺五肽。L-肌肽在60.96 μg·mL−1范围内呈线性。在0.5 ~ 60.96 μg·mL−1浓度范围内，线性关系良好。肌肽和内标物的日内和日间精密度的相对标准偏差均小于12%，回收率均大于90%。我们成功地将此方法应用于糖尿病大鼠和健康对照大鼠心肌内源性肌肽的分析。糖尿病大鼠组肌肽浓度显著低于健康对照组。这些结果支持该方法作为定量肌肽的手段的有用性，并说明L-肌肽在心肌中的重要作用。
A novel method for quantitation of cardiac muscle carnosine levels using HPLC-UV is described. In this simple and reliable method, carnosine from the rat cardiac muscle and the internal standard, thymopentin, were extracted by protein precipitation with acetonitrile. The method was linear up to 60.96 μg·mL−1 for L-carnosine. The calibration curve was linear in concentration ranges from 0.5 to 60.96 μg·mL−1. The relative standard deviations obtained for intra- and interday precision were lower than 12% and the recoveries were higher than 90% for both carnosine and internal standard. We successfully applied this method to the analysis of endogenous carnosine in cardiac muscle of the diabetes rats and healthy control rats. The concentration of carnosine was significantly lower in the diabetes rats group, compared to that in the healthy control rats. These results support the usefulness of this method as a means of quantitating carnosine and illustrate the important role of L-carnosine in cardiac muscle.