Reduction of oxidative cellular damage by overexpression of the thioredoxin TRX2 gene improves yield and quality of wine yeast dry active biomass.
Reduction of oxidative cellular damage by overexpression of the thioredoxin TRX2 gene improves yield and quality of wine yeast dry active biomass.
复制标题
DOI：
10.1186/1475-2859-9-9
复制
发表时间：
2010-02-12
影响因子：
6.4
通讯作者：
Matallana E
中科院分区：
文献类型：
--
作者：
Gómez-Pastor R;Pérez-Torrado R;Cabiscol E;Ros J;Matallana E
适应厌氧发酵的酿酒酵母菌株在活性干酵母生产的工业过程中，在好氧条件下进行生物质繁殖时遭受氧化应激。糖的氧化代谢有利于高生物量产量，但也会增加细胞成分的氧化损伤。编码硫氧还蛋白的TRX2基因的过表达增强了葡萄酒酵母菌株模型的氧化应激抗性。硫氧还蛋白和谷胱甘肽/谷胱甘肽系统构成了最重要的抗氧化防御。Trx2p还参与调控yap1p驱动的针对某些活性氧的转录反应。工业活性干生物质生产过程的实验室规模模拟表明，TRX2过表达增加了酒酵母的最终生物质产量，并在分批和补料阶段增加了其发酵能力。用改良菌株进行的微发酵显示出快速启动表型，这源于其增强的发酵能力，也增加了有益香气化合物的含量。修饰菌株显示Yap1p调控基因和其他氧化应激相关基因的转录反应增加。Sod1p、Sod2p和过氧化氢酶等抗氧化酶的活性也增强。因此，在修饰菌株中观察到脂质和蛋白质的氧化减少，这可以解释过表达硫氧还蛋白菌株的性能改善。我们报告了在葡萄酒酵母菌株中过表达硫氧还蛋白基因TRX2的几个有益效果。我们发现该菌株呈现出增强的氧化还原防御。提高TRX2过表达菌株的生物质生产过程的产量可以在几个工业应用中特别感兴趣。
Wine Saccharomyces cerevisiae strains, adapted to anaerobic must fermentations, suffer oxidative stress when they are grown under aerobic conditions for biomass propagation in the industrial process of active dry yeast production. Oxidative metabolism of sugars favors high biomass yields but also causes increased oxidation damage of cell components. The overexpression of the TRX2 gene, coding for a thioredoxin, enhances oxidative stress resistance in a wine yeast strain model. The thioredoxin and also the glutathione/glutaredoxin system constitute the most important defense against oxidation. Trx2p is also involved in the regulation of Yap1p-driven transcriptional response against some reactive oxygen species. Laboratory scale simulations of the industrial active dry biomass production process demonstrate that TRX2 overexpression increases the wine yeast final biomass yield and also its fermentative capacity both after the batch and fed-batch phases. Microvinifications carried out with the modified strain show a fast start phenotype derived from its enhanced fermentative capacity and also increased content of beneficial aroma compounds. The modified strain displays an increased transcriptional response of Yap1p regulated genes and other oxidative stress related genes. Activities of antioxidant enzymes like Sod1p, Sod2p and catalase are also enhanced. Consequently, diminished oxidation of lipids and proteins is observed in the modified strain, which can explain the improved performance of the thioredoxin overexpressing strain. We report several beneficial effects of overexpressing the thioredoxin gene TRX2 in a wine yeast strain. We show that this strain presents an enhanced redox defense. Increased yield of biomass production process in TRX2 overexpressing strain can be of special interest for several industrial applications.
登录
查看更多内容
影响因子：
3.6
作者：
Garrido, EO;Grant, CM
通讯作者：
Grant, CM
影响因子：
6.1
作者：
Hamada, Yasuhiro;Miyata, Satoshi;Kasuga, Masato
通讯作者：
Kasuga, Masato
影响因子：
2.9
作者：
GRIFFITH, OW
通讯作者：
GRIFFITH, OW
影响因子：
2.6
作者：
GIETZ, RD;SCHIESTL, RH;WOODS, RA
通讯作者：
WOODS, RA
影响因子：
5.3
作者：
Kuge, S;Arita, M;Nomoto, A
通讯作者：
Nomoto, A