Small family, big impact: In silico analysis of DREB2 transcription factor family in rice
Small family, big impact: In silico analysis of DREB2 transcription factor family in rice
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DOI：
10.1016/j.compbiolchem.2016.10.012
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发表时间：
2016-12-01
影响因子：
3.1
通讯作者：
Herath, Venura
中科院分区：
文献类型：
--
作者：
Herath, Venura
DREB蛋白被认为是植物对干旱、盐碱和低温等非生物胁迫反应的主要调节因子。它们还参与其他发育过程，如胚和胚乳发育。DREB转录因子家族由CBF 1/DREB 1和DREB 2两个亚家族组成。本研究采用全基因组电子计算机分析技术对DREB 2家族转录因子在水稻基因组中的结构和功能进行了研究。利用拟南芥DREB 2序列，共鉴定了5个水稻DREB 2同源物，它们分布在4条染色体上。所有OsDREB均含有DREB 2家族特有的AP 2结构域和[K/R]GICKGPxN基序。在水稻生长发育过程中，OsDREB 2A、OsDREB 2B和OsABI 4三种OsDREB 2基因均表达，且表达范围仅限于胚和胚乳组织。OsDREB 2A、OsDREB 2B和OsDREB 2C在非生物胁迫条件下表达。OsDREB 2B在干旱、盐胁迫和低温胁迫条件下表达，OsDREB 2A和OsDREB 2C仅在干旱和盐胁迫条件下表达。OsDREB 2的推定启动子区域富含与细胞发育、激素调节和应激反应相关的元件，验证了所观察到的表达动态。共表达分析表明，OsDREB 2s与胚胎发育和胁迫相关基因共同表达。预测的翻译后修饰表明OsDREB 2s的精细调控。这些发现可能揭示复杂的非生物胁迫信号网络和未来的基因组学研究针对气候准备作物的发展。(C)2016爱思唯尔有限公司版权所有
Dehydration-responsive element- (DREB) proteins are considered as the master regulators of plant abiotic stress responses including drought, salinity and cold. They are also involved in other developmental processes such as embryo and endosperm development. DREB family of transcription factors consist of two sub families namely CBF1/DREB1 and DREB2. In this study, a genome-wide in silico analysis was carried out to dissect the structure and function of DREB2 family transcription factors in the rice genome. Using Arabidopsis DREB2 sequences a total of five rice DREB2 homologs were identified and they were distributed among four chromosomes. All OsDREBs contained the AP2 domain and unique [K/R]GICKGPxN motif characteristic to DREB2 family. During rice growth and development, three OsDREB2s namely OsDREB2A, OsDREB2B and OsABI4 were expressed and their expression was confined to embryo and endosperm tissues. OsDREB2A, OsDREB2B and OsDREB2C were expressed under abiotic stress conditions. OsDREB2B was expressed under drought, salinity and cold stress conditions while OsDREB2A and OsDREB2C were expressed only under drought and salinity conditions. The putative promoter regions of OsDREB2s were enriched with elements related to cellular development, hormonal regulation and stress response validating the observed expression dynamics. Co-expression analysis revealed that embryo development and stress related genes were expressed together with OsDREB2s. Predicted post translational modifications indicated the fine regulation of OsDREB2s. These findings may shed light in uncovering the complex abiotic stress signaling networks and future genomics studies targeting the development of climate ready crops. (C) 2016 Elsevier Ltd. All rights reserved.