Manganese improved Trachinotus ovatus immune against Cryptocaryon irritans infection
Manganese improved Trachinotus ovatus immune against Cryptocaryon irritans infection
复制标题
DOI：
10.1016/j.aquaculture.2023.739835
复制
发表时间：
2023
期刊：
影响因子：
4.5
通讯作者：
Huicheng Wu;Xueli Lai;Wenjie Guo;Xiong Li;Yingtong Hu;X. Dan;Yanwei Li;Z. Mo
中科院分区：
文献类型：
--
作者：
Huicheng Wu;Xueli Lai;Wenjie Guo;Xiong Li;Yingtong Hu;X. Dan;Yanwei Li;Z. Mo
锰是一种必需的微量矿物质元素，据报道可以促进哺乳动物的免疫系统。然而，它在鱼类免疫中的作用还不清楚。研究Mn 2+在卵形鲳鲹抗刺激核虫免疫保护中的作用。单核细胞吞噬功能增强，检测到Mn 2+处理的ToHKL中I-IFN（IFNα3、IFNα4）、细胞因子（IL-6、TNFα）和免疫球蛋白基因（IgM、IgT）的表达水平增加。此外，我们还制备了一种分散锰的纳米材料--胶体锰盐（Mn jelly，MnJ），其对C的免疫保护率为21.0%。刺激物被注射到鲳鲹体内。与传统的佐剂弗氏佐剂相比，皮下注射MnJ后，未观察到死亡率或严重炎症。MnJ + Tt免疫小鼠，对C. MnJ + Tt免疫后，血清和脾培养液中特异性IgM和IgM阳性信号增加。此外，T. ovatuscGAS（TocGAS）被克隆并鉴定了关键的保守残基，这些残基表明锰通过激活cGAS分子来改善鲳免疫力。提示锰可提高鲳免疫力。MnJ可能是一种很有前途佐剂候选物。刺激物疫苗然而，需要进一步的研究来确定TocGAS是否被锰激活。
Manganese is an essential trace mineral element that has been reported to promote mammalian immune systems. However, its role in fish immunity is not well understood. This study aimed to evaluate the function of Mn2+inTrachinotus ovatusimmune protection againstCrytocaryon irritans. Monocyte phagocytosis was enhanced and increased expression levels of I-IFNs (IFNα3, IFNα4), cytokines (IL-6, TNFα), and immunoglobulin genes (IgM, IgT) were detected in ToHKLs treated with Mn2+. Additionally, we prepared colloidal manganese salt (Mn jelly, MnJ), a nano-material with dispersed manganese, which provided 21.0% immune protection againstC. irritanswhen injected into pompano. Compared to traditional adjuvant Freund's adjuvant, no mortality or intense inflammation was observed in pompano after subcutaneous injection of MnJ. Immunization with MnJ plusTetrahymena thermophila(MnJ + Tt) resulted in better immune protection againstC. irritanschallenge, and an increase in specific IgMs in serum and spleen culture medium as well as IgM positive signal were found in MnJ + Tt-vaccinated pompano. Furthermore,T. ovatuscGAS (TocGAS) was cloned and identified critical conserved residues that suggest manganese improves pompano immunity by activating the cGAS molecule. Our results indicate that manganese could improve pompano immunity againstC. irritansinfection and that MnJ may be a promising adjuvant candidate forC. irritansvaccine. However, further studies are needed to determine whether TocGAS is activated by manganese.