LYSIS OF GRAM-NEGATIVE BACTERIA BY HOST-INDEPENDENT ECTOPARASITIC BDELLOVIBRIO BACTERIOVORUS ISOLATES
LYSIS OF GRAM-NEGATIVE BACTERIA BY HOST-INDEPENDENT ECTOPARASITIC BDELLOVIBRIO BACTERIOVORUS ISOLATES
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DOI：
10.1099/00221287-40-3-317
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发表时间：
1965-01-01
期刊：
影响因子：
--
通讯作者：
BRUFF, B
中科院分区：
文献类型：
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作者：
SHILO, M;BRUFF, B
描述了能够在无宿主培养基上生长的卵形芽孢杆菌菌株的分离方法和营养需求。这些菌株在无宿主培养基中多次转移后，仍保留了对各种革兰氏阴性细菌的寄生能力。在寄生虫+宿主悬浮液中，各种蛭弧菌菌株的宿主特异性谱比在宿主草坪上检查斑块得到的谱要宽得多。因此，生长条件影响蛭弧菌附着和溶解宿主生物的能力。由某些蛭弧菌菌株形成的蛋白水解外酶可以消化热杀、酸处理或edta处理的宿主生物，但不影响完整的宿主生物。高磷酸盐浓度抑制外泌酶活性。形成外泌酶的寄生弧菌菌株诱导裂解的形态序列表明，这种裂解是一个两阶段的过程：第一阶段是蛭弧菌特异性附着并破坏宿主细胞壁；第二阶段是由蛭弧菌产生的外酶对细胞成分的非特异性消化。
Methods of isolation and nutritional requirements of B. bacteriovorus strains capable of growth on host-free media are described. Such strains retained their parasitic capacities for various gram-negative bacteria after many transfers in host-free media. In parasite + host suspensions the spectrum of host specificity of various bdellovibrio strains was considerably wider than that obtained by examination for plaques on host lawns. The growth conditions therefore affect the capacity of the bdellovibrios to attach to and lyse host organisms. A proteolytic exoenzyme formed by certain bdellovibrio strains digested heat-killed, acid-treated or EDTA-treated host organisms, but did not affect intact host organisms. High phosphate concentrations inhibited exoenzyme activity. The morphological sequence of lysis induced by exoenzyme-forming parasitic bdellovibrio strains suggested that this lysis was a two-stage process: the first stage was specific attachment of bdellovibrios with damage to the cell wall of the host organisms; the second stage was non-specific digestion of cellular components by exoenzyme produced by the bdellovibrios.