Light-controlled twister ribozyme with single-molecule detection resolves RNA function in time and space
Light-controlled twister ribozyme with single-molecule detection resolves RNA function in time and space
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DOI：
10.1073/pnas.2003425117
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发表时间：
2020-06-02
影响因子：
11.1
通讯作者：
Woodson, Sarah A.
中科院分区：
文献类型：
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作者：
Korman, Arthur;Sun, Huabing;Woodson, Sarah A.
小的核酶，如水稻扭转酶，当核酶折叠成活性构象时，会自发地切割它们自己的RNA。然而，扭转折叠和自切割之间的耦合一直难以研究，因为活性核酶迅速转化为产物。在这里，我们描述了一个photocaged核苷酸的合成，释放鸟苷在微秒后，用蓝光光溶剂分解。将该工具应用于O. sativa twister实现了空间（75 μ m）和时间（
Small ribozymes such as Oryza sativa twister spontaneously cleave their own RNA when the ribozyme folds into its active conformation. The coupling between twister folding and self-cleavage has been difficult to study, however, because the active ribozyme rapidly converts to product. Here, we describe the synthesis of a photocaged nucleotide that releases guanosine within microseconds upon photosolvolysis with blue light. Application of this tool to O. sativa twister achieved the spatial (75 mu m) and temporal (