Discovery and functional understanding of MiRNAs in molluscs: a genome-wide profiling approach
Discovery and functional understanding of MiRNAs in molluscs: a genome-wide profiling approach
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软体动物中 miRNA 的发现和功能理解：全基因组分析方法
DOI：
10.1080/15476286.2020.1867798
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发表时间：
2021-01-09
期刊：
影响因子：
4.1
通讯作者：
Asakawa, Shuichi
中科院分区：
文献类型：
--
作者：
Huang, Songqian;Yoshitake, Kazutoshi;Asakawa, Shuichi
小非编码 RNA 在各种生物体的基因调控、转座元件抑制和病毒活性中发挥着关键作用。在这些小非编码 RNA 的各个类别中，microRNA (miRNA) 指导细胞发育、增殖、凋亡、肿瘤发生和分化中的翻译后基因调控。在这里，我们对 miRNA 进行了全基因组计算预测，以提高对软体动物中 miRNA 观察和功能的理解。作为第一步，通过基因组扫描预测了 35 个软体动物物种中数百个保守的 miRNA。随后，对 miRNA 的种群、亚型、组织和功能进行了详细表征。此外，基于同源序列搜索，阐明了关键的 miRNA 生物合成因子，包括 AGO2、DGCR8、DICER、DROSHA、TRABP2、RAN 和 XPO5。我们还总结了 miRNA 在生物矿化、免疫和应激反应以及软体动物生长和发育中的功能。由于 miRNA 在各种生命形式中发挥着至关重要的作用，因此这项研究将深入了解软体动物以及其他无脊椎动物中的 miRNA 生物发生和功能。
Small non-coding RNAs play a pivotal role in gene regulation, repression of transposable element and viral activity in various organisms. Among the various categories of these small non-coding RNAs, microRNAs (miRNAs) guide post-translational gene regulation in cellular development, proliferation, apoptosis, oncogenesis, and differentiation. Here, we performed a genome-wide computational prediction of miRNAs to improve the understanding of miRNA observation and function in molluscs. As an initial step, hundreds of conserved miRNAs were predicted in 35 species of molluscs through genome scanning. Afterwards, the miRNAs' population, isoforms, organization, and function were characterized in detail. Furthermore, the key miRNA biogenesis factors, including AGO2, DGCR8, DICER, DROSHA, TRABP2, RAN, and XPO5, were elucidated based on homologue sequence searching. We also summarized the miRNAs' function in biomineralization, immune and stress response, as well as growth and development in molluscs. Because miRNAs play a vital role in various lifeforms, this study will provide insight into miRNA biogenesis and function in molluscs, as well as other invertebrates.