Integrated membrane filters for minimizing hydrodynamic flow and filtering in microfluidic devices.
Integrated membrane filters for minimizing hydrodynamic flow and filtering in microfluidic devices.
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DOI：
10.1021/ac070943f
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发表时间：
2007-08
影响因子：
7.4
通讯作者：
S. Noblitt;James R. Kraly;Jaimie M VanBuren;S. Hering;J. Collett;C. Henry
中科院分区：
文献类型：
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作者：
S. Noblitt;James R. Kraly;Jaimie M VanBuren;S. Hering;J. Collett;C. Henry
由于有可能开发便携式、廉价的分析工具，能够以较低的样品消耗量进行快速分析，因此微流控设备已经获得了重大的科学兴趣。这些特性使微流控设备在传统技术功能有限的使用点测量中具有吸引力。然而，许多生物和环境分析中感兴趣的样品含有可能堵塞微通道的不溶颗粒，分析前的人工过滤对于使用点应用是不可取的。类似地，有些情况涉及对样品体积的有限控制，可能会由于不同的流体压头而导致不需要的流体动力流动。在这里，我们提出了在聚二甲基硅氧烷毛细管电泳微芯片储存库中成功地包含径迹刻蚀聚碳酸酯膜过滤器。结果表明，该膜具有较好的过滤不溶性颗粒的能力，其选择性取决于膜孔径。用含膜微芯片对阳离子、阴离子和氨基酸进行了电泳分离，并用电导和荧光检测进行了监测。在门控进样中，峰面积对头部压力的依赖性最高可降低92%。结果表明，膜的加入不会影响膜的分离性能。将膜结合到微流控设备的储存库中将允许改进对复杂溶液和体积控制不佳的样品的分析。
Microfluidic devices have gained significant scientific interest due to the potential to develop portable, inexpensive analytical tools capable of quick analyses with low sample consumption. These qualities make microfluidic devices attractive for point-of-use measurements where traditional techniques have limited functionality. Many samples of interest in biological and environmental analysis, however, contain insoluble particles that can block microchannels, and manual filtration prior to analysis is not desirable for point-of-use applications. Similarly, some situations involve limited control of the sample volume, potentially causing unwanted hydrodynamic flow due to differential fluid heads. Here, we present the successful inclusion of track-etched polycarbonate membrane filters into the reservoirs of poly(dimethylsiloxane) capillary electrophoresis microchips. The membranes were shown to filter insoluble particles with selectivity based on the membrane pore diameter. Electrophoretic separations with membrane-containing microchips were performed on cations, anions, and amino acids and monitored using conductivity and fluorescence detection. The dependence of peak areas on head pressure in gated injection was shown to be reduced by up to 92%. Results indicate that separation performance is not hindered by the addition of membranes. Incorporating membranes into the reservoirs of microfluidic devices will allow for improved analysis of complex solutions and samples with poorly controlled volume.