Genome-wide identification and characterization of the aquaporin gene family in Medicago truncatula
Genome-wide identification and characterization of the aquaporin gene family in Medicago truncatula
复制标题
蒺藜苜蓿水通道蛋白基因家族的全基因组鉴定和表征
DOI：
10.1007/s13562-018-0484-4
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发表时间：
2019-09-01
影响因子：
1.9
通讯作者：
Liu, Wenxian
中科院分区：
文献类型：
--
作者：
Min, Xueyang;Wu, Honglin;Liu, Wenxian
水通道蛋白（Aquaporins，AQP）又称主要内在蛋白（Major Intrinsic Protein，MIPs），是植物中一个庞大而多样的蛋白通道转运蛋白家族。然而，关于模式豆科植物蒺藜苜蓿中AQP家族的信息仍然知之甚少。在这里，46个AQPs被确定和表征。系统发育分析表明，这些基因分属于5个亚家族，分别为10个质膜内在蛋白，12个液泡膜内在蛋白，18个NOD 26-like内在蛋白，4个小碱性内在蛋白和2个未知内在蛋白。系统分析了水通道蛋白的亚细胞定位、基因结构、染色体定位、进化应激和功能残基等基本信息。此外，基因表达谱的计算机分析表明，某些AQP基因具有一定的组织特异性和对干旱/盐胁迫的响应性。qRT-PCR结果表明，MtPIP 1;5和MtTIP 1;3在干旱和盐胁迫条件下均显著上调，表明它们在响应非生物胁迫中发挥重要作用。本研究结果为进一步研究M.蒺藜和相关的豆科植物。
Aquaporins (AQPs), or major intrinsic proteins (MIPs), constitute a large and diverse family of protein channel transporter in plants. However, the information about AQPs family is still poorly understood in the model legume species Medicago truncatula. Here, 46 AQPs were identified and characterized. Based on the phylogenetic analysis, these genes were assigned to five subfamilies, specifically as 10 plasma membrane intrinsic proteins, 12 tonoplast intrinsic proteins, 18 NOD26-like intrinsic proteins, 4 small basic intrinsic proteins and 2 uncharacterized intrinsic proteins. The essential information of AQPs, such as subcellular localization, gene structure, chromosomal localization, evolutionary stress and functional residues were systematically analyzed. In addition, in silico analysis of the gene expression profiles showed certain of tissue specificity and responsiveness to drought/salt stresses in some AQPs genes. The qRT-PCR results showed that MtPIP1;5 and MtTIP1;3 were significantly up-regulated under both drought and salinity stress conditions, indicated their important roles in response to abiotic stresses. The results could provide valuable information for the further functional analysis of the AQPs in M. truncatula and related legume species.