Reconstitution of the early steps of gliotoxin biosynthesis in Aspergillus nidulans reveals the role of the monooxygenase GliC.
Reconstitution of the early steps of gliotoxin biosynthesis in Aspergillus nidulans reveals the role of the monooxygenase GliC.
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DOI：
10.1016/j.bmcl.2013.01.099
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发表时间：
2013-04-01
影响因子：
2.7
通讯作者：
Wang, Clay C. C.
中科院分区：
文献类型：
--
作者：
Chang, Shu-Lin;Chiang, Yi-Ming;Yeh, Hsu-Hua;Wu, Tung-Kung;Wang, Clay C. C.
胶质毒素是表聚硫代二氧代哌嗪（epipolythiodioxopiperazine，ETP）的一种，因其广泛的生物活性而受到科学界的广泛关注。然而，尽管鉴定了胶粘毒素簇，但胶粘毒素生物合成中的步骤顺序仍然难以捉摸。作为迄今为止使用的基因敲除和生物化学方法的替代，在这里，我们报告使用异源表达方法来确定A. nidulans。GliC是一种单加氧酶，参与胶质毒素生物合成途径的第二步，催化l-苯丙氨酸α位羟基化。
The gliotoxin, a member of the epipolythiodioxopiperazine (ETP), has received considerable attention from the scientific community for its wide range of biological activity. Despite the identification of gliotoxin cluster, however, the sequence of steps in the gliotoxin biosynthesis has remained elusive. As an alternative to the gene knock out and biochemical approaches used so far, here we report using a heterologous expression approach to determine the sequence of the early steps of gliotoxin biosynthesis in A. nidulans. We identified the GliC, a monooxygenases that involved in the second step of gliotoxin biosynthesis pathway through the catalyzing the hydroxylation at the α-position of l-Phe.
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影响因子：
6.7
作者：
Schrettl M;Carberry S;Kavanagh K;Haas H;Jones GW;O'Brien J;Nolan A;Stephens J;Fenelon O;Doyle S
通讯作者：
Doyle S
影响因子：
15
作者：
Forseth, Ry R.;Fox, Ellen M.;Chung, DaWoon;Howlett, Barbara J.;Keller, Nancy P.;Schroeder, Frank C.
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Schroeder, Frank C.
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通讯作者：
EICHNER, RD
影响因子：
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作者：
Scharf, Daniel H.;Remme, Nicole;Hertweck, Christian
通讯作者：
Hertweck, Christian